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Importance of human β and Endothelial cell Cooperation for the maturation, organisation and functionality of islet organoids.

Objective

To cure diabetes, functional ß cell mass must be restored. Transplantation of human islets has seen significant progress over the past decades. However, organ donor shortage is a major obstacle. For transplanted patients, poor graft revascularisation and a recurrent autoimmune attack ultimately result in the loss of the graft. Human induced pluripotent stem cell (iPSC)-derived ß cells are a promising alternative source of cells that is inexhaustible and allows genetic interventions prior to transplantation to stimulate revascularisation, immunoprotection and ultimately graft survival. iPSC-ß cells retain an immature phenotype in vitro and only in vivo acquire mature function equivalent to that of native human islets. Intra-islet endothelial cells (ECs) form a necessary niche for pancreatic endocrinogenesis and adult ß-cell function, and they improve islet survival after transplantation. This proposal aims to test the hypothesis that ECs are crucial to generate mature and functional human iPSC-derived islet organoids in vitro and to promote revascularisation, function, adaptation to metabolic challenges and survival in vivo. To this end, we will generate 'vascularised' islet organoids containing iPSC-derived endocrine and ECs. By combining genetic interventions and cutting-edge technologies, such as whole tissue three-dimensional analysis and transcriptomics, we will characterise the impact of ECs on islet organoids. This project will broaden the knowledge on the essential relationship linking structure to function in human islet organoids, paving the way for new human cell therapies.

Fields of science (EuroSciVoc)

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Keywords

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITE LIBRE DE BRUXELLES
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 175 920,00
Address
AVENUE FRANKLIN ROOSEVELT 50
1050 Bruxelles / Brussel
Belgium

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Region
Région de Bruxelles-Capitale/Brussels Hoofdstedelijk Gewest Région de Bruxelles-Capitale/ Brussels Hoofdstedelijk Gewest Arr. de Bruxelles-Capitale/Arr. Brussel-Hoofdstad
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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