Project description
Advanced point-of-care cytokine detection
Protein biomarkers in biological samples have been widely used to indicate the presence, progression or response to the treatment of a disease. Their detection and quantification traditionally rely on time-consuming and costly techniques such as enzyme-linked immunosorbent assays (ELISA), mass spectrometry or immunohistochemistry. With the support of the Marie Skłodowska-Curie Actions programme, the RESPONSE project aims to develop innovative point-of-care biosensors for cytokine detection. The research team will combine special materials and catalysts that enhance the capture of these molecules and the measurable signal, making the sensor more sensitive and easier to use.
Objective
Cytokines, such as TNF-α, hold immense significance as protein biomarkers, pivotal for diagnostic, prognostic, and therapeutic management across various clinical disorders like Alzheimer’s disease, sepsis, and cancer. Nonetheless, prevailing techniques like enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (PCR) prove to be expensive, time-intensive, and reliant on specialized personnel, intricate instrumentation, and infrastructure. Furthermore, the performance of present biosensing techniques necessitates refinements in terms of real-time outcomes, sensitivity, affordability, and user-friendliness. Within this context, the RESPONSE project sets out to forge an innovative approach, intertwining Janus graphene derivatives (JGDs) and Single Atom Catalysts (SACs), or atozymes, for Point-Of-Care (POC) biosensors for cytokine detection. This undertaking involves the strategic utilization of selectively and densely functionalized JGDs, serving dual roles: i) as signal transducer immobilized on a paper lateral flow electrochemical sensor (LFES), and carrying the cytokine bioreceptor (primary/capture antibody; Ab1) and ii) as signal amplifier JGDs, housing the SACs and the secondary/signal antibody (Ab2), which binds to a different epitope of cytokine. The binding between TNF-α and JGDs-Ab1 initiates the sandwich formation with SAC-JGDs-Ab2 complex at the test region. Here, the SACs catalyze the oxidation of an appropriate reporter molecule, liberating electrons and thereby significantly amplifying the signal output which can be quantified through an integrated electrode system. REPSONSE offers affordability, point of care readiness and commercialization potential. Importantly, RESPONSE’s strategy defines the blueprint for transitioning from the current, symptom-focused and late-stage diagnosis to the next-generation prognostic biomarker-guided preventative or therapeutic treatment management, and improve life-quality of millions of people worldwide.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology nanotechnology nano-materials two-dimensional nanostructures graphene
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences catalysis
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
771 47 Olomouc
Czechia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.