Project description
Molecular transport in plant roots
Plants contain a hydrophobic barrier that helps prevent the movement of water and solutes across the cell walls, contributing to the impermeability of certain tissues such as the endodermis in plant roots. The deposition of this hydrophobic substance occurs through a process called suberisation which plays a central role in the regulation of ion and water uptake from the soil. Funded by the Marie Skłodowska-Curie Actions programme, the SyDy RoCe project aims to study the dynamics of plant transport systems under stress conditions, refining models of root function. Researchers will manipulate specific cells in the endodermis of plants and use specific dyes to investigate the exchange of molecules in the plant vascular system.
Objective
Roots must tightly balance uptake of ions and water from the soil into their vasculature. For this purpose, they developed complex systems of barrier mechanisms. The endodermis layer encircles the vasculature and via the casparian strip (CS) and suberin acts as such a barrier. The CS blocks flux outside of cells (the apoplastic flux), and leaves only the symplastic pathway open for the exchange of molecules. Endodermis cell walls undergo suberisation dependent on phloem pole (PP) or xylem pole (XP) association. The unsuberised XP associated endodermis (XPAE) cells are speculated to fulfil an important role as transport highways. Despite this close association to the underlying vasculature, little is known about the dynamics of endodermal symplastic flux in the different poles.
I aim to elucidate differences in the cell-to-cell exchange of the endodermis in relation to barrier status and radial positioning. For this, I will use a photoconvertible fluorophore to quantify symplastic flux by XP versus PP association and suberisation status. Next, I will generate tools to specifically manipulate XPAE cells, by forcing them to close off their symplastic connections by plasmodesmal callose deposition, or to undergo suberisation.
I will use these tools to assess phenotypic differences on plant growth under stress conditions that affect endodermal barrier development (e.g. low iron, phosphate, and zinc). Together, this will result in unprecedented insights into how the endodermis achieves the specific transport of water, and ions, and will allow me to update models with radial information on endodermal selectivity and connection to vascular transport.
Fields of science (EuroSciVoc)
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CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
80539 MUNCHEN
Germany
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