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Rational Design of ACAT Single Enzyme Nanogels for Hepatic Fibrosis Treatment-NanoACATre

Project description

Single Enzyme Nanogels for hepatic fibrosis management

Hepatic fibrosis is a progressive process in which functional liver tissue is gradually replaced by an extracellular matrix rich in highly cross-linked collagen, with hepatic stellate cells playing a vital role in this process. With the support of the Marie Skłodowska-Curie Actions programme, the NanoACATre project aims to develop new Single Enzyme Nanogels (SENs) to manage cholesterol levels in hepatic stellate cells, preventing their activation and associated complications such as hepatocellular carcinoma. The project will treat hepatic fibrosis by enhancing intracellular Acyl-CoA:Cholesterol Acyltransferase (ACAT) in these cells and will use advanced techniques to prepare and characterise ACAT-SENs, evaluating their impact on ACAT delivery in a rat hepatic stellate cell line.

Objective

Hepatic fibrosis is a dynamic process characterized by the progressive replacement of functional liver tissue by an extracellular matrix rich in highly cross-linked collagen, with hepatic stellate cells being the main actors of this process. NanoACATre, intended for the treatment for hepatic fibrosis based on augmenting intracellular Acyl-CoA:Cholesterol Acyltransferase (ACAT) in hepatic stellate cells, brings together a talented researcher (Dr. Andoni Rodriguez-Abetxuko), with a background in nanomaterials and biocatalysis, and iNANO-Aarhus University, an innovative and multidisciplinary research institute at the forefront of nanohybrid-based biomedical solutions for liver disease. NanoACATre will focus on advancing the scientific career of Dr. Rodriguez-Abetxuko through the development of new Single Enzyme Nanogels (SENs) for the successful management of cholesterol homeostasis in activated hepatic stellate cells to prevent or reconvert their activation and prevent associated complications such as hepatocellular carcinoma. Under the supervision of Prof. Brigitte Stdler, a globally recognized researcher on the field of bottom-up synthetic biology, NanoACATre will proceed in a stepwise fashion using cutting-edge methodologies to prepare and physiochemically characterize ACAT-SENs. The effect of the ACAT-SEN surface physicochemical properties will be evaluated on the ACAT delivery in a rat hepatic stellate cell line. The interdisciplinary work, integrating expertise in polymeric nanogels and protein delivery, will be necessary to achieve NanoACATre's main goal, and the Cell Mimicry Lab, led by Prof. Stadler, is the ideal place to turn this into reality. NanoACATre is designed to broaden the scientific knowledge and transferable skills of Dr. Rodriguez-Abetxuko. In addition, NanoACATre will expand his professional network on the way to eventually becoming an independent group leader in the field of nanomedicine.

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Topic(s)

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

AARHUS UNIVERSITET
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 214 934,40
Address
NORDRE RINGGADE 1
8000 Aarhus C
Denmark

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Region
Danmark Midtjylland Østjylland
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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