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Mechanisms of Soil Restructuring by Bacterial Aggregates: towards a more efficient and resilient Agriculture

Project description

Roles of bacteria in soil aggregation

Soil bacteria are thought to play a key role in forming organo-mineral aggregates, which support plant health and improve soil stability. Restoring aggregates in damaged soils could improve the function of soils and thereby increase crop resistance to extreme weather and parasites. With the support of the Marie Skłodowska-Curie Actions programme, the AgriGate project aims to acquire a fundamental understanding of how bacteria form aggregates in soil. It will employ model laboratory systems recreating microscopic environments where the interactions between bacteria and soil particles can be closely studied. In particular, the roles of soil-particle size, abundance of bacterial resources, and polymer secretion by bacteria will be investigated. The acquired biophysical knowledge will guide future design of biological remediation methods for soils.

Objective

Soil bacteria are of critical importance to soil health. Soil pore-size distribution over multiple length scales, which facilitates nutrient transport, depends on the presence of soil aggregates, cohesive organo-mineral assemblies formed by bacterial activity. Aggregates support plant health and increase soil stability. Although restoring aggregate structure in degraded soil can yield great benefits for extreme-weather resilience and agricultural productivity, we lack any mechanistic understanding of how aggregates are formed by bacteria. This knowledge gap hampers the deployment of promising bio-augmentation strategies for soil restoration, such as inoculation of soil with aggregate-promoting bacteria. The objective of AgriGate is to narrow this gap by elucidating the biophysical mechanisms of bacterial aggregate-formation. I will focus on the reciprocal mechanical interactions between bacteria and soil grains, using interdisciplinary methods over multiple spatial scales. I will first develop a novel microfluidic chip granting real-time optical access to bacterial dynamics inside a three-dimensional bed of model soil grains. AgriGate’s hypotheses are that (1) there exists an optimal grain size and inoculant richness for bacteria-led aggregation, and (2) that bacterial extracellular polymeric substances (EPS) are necessary for aggregation. I will test these hypotheses by varying grain size, inoculant richness, and bacterial EPS-production in the chip. Microfluidic experiments will be combined with experiments in bacteria-loaded soil columns observed by X-ray tomography, to extend results to the macroscopic scale and link microscale structure to macroscale function. The search for optimal aggregation conditions will be formalized mechanistically and extended by a numerical model of bacterial colony growth in a cohesive granular medium. Together, AgriGate’s new tools and results will provide a strong basis for fundamental and applied studies on soil bio-augmentation.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITE DE RENNES
Net EU contribution

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€ 195 914,88
Address
263 AVENUE DU GENERAL LECLERC
35042 RENNES
France

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Activity type
Higher or Secondary Education Establishments
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Total cost

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