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From RNA-peptide coevolution to cellular life at heated air bubbles

Project description

Investigating the Darwinian evolution of RNA and peptides

The origin of life on Earth remains a mystery. Recently, it was discovered that heat-exposed air bubbles promote molecular evolution, facilitating the cooperation of amino acids, nucleosides, and lipids. This environment encourages RNA oligomerisation and the formation of ribozymes. Lipids create vesicles that encapsulate RNA, while amino acids form peptides, leading to RNA-peptide hybrids and early proto-translation. The ERC-funded BubbleLife project will investigate the Darwinian evolution of RNA and peptides in a controlled environment with limited starting molecules. The project will conduct focused experiments that connect amino acids and nucleotides to the emergence of proto-cellular life, aiming to create ‘proto-cell generators’ and support primitive RNA-based replicators and modern transcription-translation systems.

Objective

"The origin of life on Earth is one of humanity's profound mysteries that lacks scientific answers. We aim to answer this fundamental question by performing pioneering experiments starting from the Darwinian evolution of RNA and peptides to the emergence of cellular life, all in a single environment with only a small selection of starting molecules. We are an interdisciplinary team with diverse backgrounds in chemistry, physics, and biochemistry, and we have a proven track record of strong interdisciplinary collaborations.

Preliminary experiments have revealed the remarkable potential of heat-exposed air bubbles for molecular evolution. BubbleLife connects long evolutionary timescales through targeted experiments, bridging from amino acids and nucleotides to the emergence of protocellular life in one unified environment. Our experimental studies indicate a chemical cooperation of amino acids, nucleoside monophosphates and lipids, driving their Darwinian co-evolution towards modern biochemistry. The non-equilibrium at heated air bubbles promotes RNA oligomerization and template-directed ligation, fostering the emergence of autocatalytic replication networks, including the first RNA catalysts (ribozymes). Co-accumulating lipids form membrane vesicles at heated air-water interfaces that autonomously encapsulate RNA. Driven by prebiotic phosphorylation, amino acids polymerize into peptides in the same alkaline environment. Subsequently, they form RNA-peptide hybrids, revealing intriguing modes of early proto-translation. By combining our unique expertises in a synergistic manner, our work will ultimately culminate in ""protocell generators"" that feed and encapsulate not only primitive RNA-based replicators, but also modern transcription-translation-based systems.

BubbleLife will reshape our understanding of the origin of life on Earth and potentially elsewhere in the universe.
"

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-ERC-SYG - HORIZON ERC Synergy Grants

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Call for proposal

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(opens in new window) ERC-2024-SyG

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Host institution

TECHNISCHE UNIVERSITAT DORTMUND
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 980 132,50
Address
AUGUST SCHMIDT STRASSE 4
44227 Dortmund
Germany

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Region
Nordrhein-Westfalen Arnsberg Dortmund, Kreisfreie Stadt
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 980 132,50

Beneficiaries (2)

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