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A completely biological and human tissue-engineered sling produced from Cell-Assembled extracellular Matrix (CAM) in vitro for the treatment of Stress Urinary Incontinence (SUI)

Project description

Developing biological slings to treat stress urinary incontinence

Stress urinary incontinence is a common condition in women characterised by the sudden, involuntary secretion of urine during activities that increase abdominal pressure. Treatment often entails implanting synthetic slings that provide mechanical support to the urethra. However, they can cause complications due to the hardness of their material and the body’s immune response to foreign objects. The ERC-funded SUITEX project aims to develop and evaluate slings made from biological textiles but with adequate mechanical properties. They will be engineered from a biomaterial called cell-assembled extracellular matrix (CAM) produced by ovine and human cells. CAM sheets can be cut and woven. An ovine CAM sling will then be tested in vivo on female sheep to justify validity for pre-clinical human studies.

Objective

Stress Urinary Incontinence (SUI) is a major medical issue affecting 1 in 5 women. Implantation of synthetic slings, made of knitted plastic monofilament, is the gold standard surgical treatment. They provide mechanical support to the urethra avoiding involuntary loss of urine on effort. However, they are associated with serious complications and some countries have already suspended their use. Hence, there is a strong medical and societal need for breakthrough innovations. The two fundamental problems of synthetic slings are the excessive hardness of the material and the foreign body reaction it creates. We propose a new paradigm by providing a non-living tissue-engineered sling that is soft, yet strong, as well as completely biological and human. Our strategy is based on a unique material: the Cell-Assembled extracellular Matrix (CAM) produced by fibroblasts in vitro. We can produce robust CAM sheets from human or ovine cells with similar properties. They are cut to produce threads subsequently woven into CAM slings.

The potential of CAM slings to treat SUI will be demonstrated through 2 main tasks:
1) Development of human and ovine CAM slings with mechanical properties justifying a preclinical study.
2) In vivo validation of allogenic CAM slings in a female sheep model at 2 and 6 months.

This PoC will:
1) Prove that human CAM slings can have clinically relevant mechanical properties;
2) Demonstrate that equivalent ovine CAM slings can be successfully implanted in a clinically relevant, allogenic, large animal model;
3) Show a superior integration of ovine CAM slings compared to synthetic slings (reduced immune response and positive remodeling).

Overall, this PoC will increase the technology readiness level of our product from 1-2 to 3-4. These outcomes will justify an expanded in vivo analysis and support a commercial endeavor targeting clinical application.

Host institution

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
Net EU contribution
€ 150 000,00
Address
RUE DE TOLBIAC 101
75654 Paris
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
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Total cost
No data

Beneficiaries (1)