Using synthetic lethality to identify functional genetic Relationships in microAlgae: The next step For systemAtic gEnetic interactions anaLysis in Chlamydomonas reinhardtii

Project Information

RAFAEL

Grant agreement ID: 101206719

DOI 10.3030/101206719

EC signature date 27 March 2025

Start date 1 July 2025

End date 30 June 2027

Funded under

• Marie Skłodowska-Curie Actions (MSCA)

Total cost No data

EU contribution € 207 758,16

Coordinated by

MIKROBIOLOGICKY USTAV AV CR V.V.I
Czechia

Objective

Genetic interactions describe the phenotypic consequences of variations in a specific gene, whether genetically or environmentally induced, associated with other gene. Discovering synthetic genetic interactions is crucial for unraveling the complexity of metabolic pathways and cellular functions, offering deeper insights into how cells operate under stress. Traditional genetic studies in C. reinhardtii have often focused on individual gene mutations, without fully exploiting its sexual reproduction capabilities to generate double mutants. This project aims to bring a new perspective to the study of gene functions in C. reinhardtii by identifying synthetic genetic interactions among nutrient metabolism genes. Through an innovative interdisciplinary approach involving the use of available single mutant strains from Chlamydomonas Library Project (CLiP) collection, established genetic tools, and the generation of haploid double mutants via crossing, this project seeks to uncover complex interactions between metabolic pathways. By serving as a foundation for future large-scale synthetic interaction studies, this project goes beyond current methodologies in studying genetic functions in C. reinhardtii, impacting the research field of metabolic regulation in photosynthetic organisms. The RAFAEL project will contribute significantly to the fellows professional development, equipping him with expertise in algal genetics and expanding his interdisciplinary research scope.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• natural sciences › biological sciences › genetics › mutation

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Keywords

• Chlamydomonas reinhardtii Synthetic-lethality Synthetic-sickness Genetic interactions Epistasis Metabolism TOR1 pathway

Programme(s)

• HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme

Topic(s)

• HORIZON-MSCA-2024-PF-01-01 - MSCA Postdoctoral Fellowships 2024

Call for proposal

HORIZON-MSCA-2024-PF-01

Funding Scheme

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

Coordinator

MIKROBIOLOGICKY USTAV AV CR V.V.I

• Net EU contribution: € 207 758,16
• Address: VIDENSKA 1083 ; 142 00 Praha 4 ; Czechia
• Region: Česko › Praha › Hlavní město Praha
• Activity type: Research Organisations
• Total cost: No data