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Using synthetic lethality to identify functional genetic Relationships in microAlgae: The next step For systemAtic gEnetic interactions anaLysis in Chlamydomonas reinhardtii

Project description

Hidden links in algal genes

Gene interaction determines how photosynthetic cells react to stress and regulate metabolism. This is not well understood, particularly for the model green alga Chlamydomonas reinhardtii, which is widely used to study photosynthesis and nutrient metabolism. Supported by the Marie Skłodowska-Curie Actions programme, the RAFAEL project aims to study genetic interactions. Specifically, it will make use of already available single mutant strains from the Chlamydomonas Library Project and create double mutants through sexual crossing. The outcome has the potential to revolutionise our understanding of metabolic control. The project will also provide professional training in algal genetics and cross-disciplinary strategies.

Objective

Genetic interactions describe the phenotypic consequences of variations in a specific gene, whether genetically or environmentally induced, associated with other gene. Discovering synthetic genetic interactions is crucial for unraveling the complexity of metabolic pathways and cellular functions, offering deeper insights into how cells operate under stress. Traditional genetic studies in C. reinhardtii have often focused on individual gene mutations, without fully exploiting its sexual reproduction capabilities to generate double mutants. This project aims to bring a new perspective to the study of gene functions in C. reinhardtii by identifying synthetic genetic interactions among nutrient metabolism genes. Through an innovative interdisciplinary approach involving the use of available single mutant strains from Chlamydomonas Library Project (CLiP) collection, established genetic tools, and the generation of haploid double mutants via crossing, this project seeks to uncover complex interactions between metabolic pathways. By serving as a foundation for future large-scale synthetic interaction studies, this project goes beyond current methodologies in studying genetic functions in C. reinhardtii, impacting the research field of metabolic regulation in photosynthetic organisms. The RAFAEL project will contribute significantly to the fellows professional development, equipping him with expertise in algal genetics and expanding his interdisciplinary research scope.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2024-PF-01

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Coordinator

MIKROBIOLOGICKY USTAV AV CR V.V.I
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 207 758,16
Address
VIDENSKA 1083
142 00 PRAHA 4
Czechia

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Region
Česko Praha Hlavní město Praha
Activity type
Research Organisations
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Total cost

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