Objective
Vascular plants have a long-distance transport system consisting of phloem and xylem. The phloem provides the path for the distribution of photosynthates and for the translocation of proteins and mRNAs involved in plant growth and development. Phloem tissu e is composed of several specialised cells. The sieve tube is the main conducting unit in the phloem tissue. Sieve tubes are highly specialised cells that are connected symplastically through sieve plates to form continuous columns. We will use a combined genetic and genomic approach to study the processes and factors that control sieve tube differentiation. We will take advantage of an Arabidopsis promoter-trap line, which shows expression of a marker gene (UidA) in loci of phloem differentiation. We have subjected this line to EMS mutagenesis and will identify and characterise mutants with altered expression patterns of the marker gene. We have also isolated the regulatory sequence that is responsible for the marker gene expression in the promoter-trap lin e and fused it to the gene for green fluorescent protein (GFP). We will use the resulting plants for the isolation of cells that undergo phloem differentiation. Subsequently, we aim to investigate the transcriptome of these cells using microarray technique s. The objective of this proposal is to investigate the factors and genes that control phloem development and to obtain a complete picture of phloem cell differentiation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA transcriptomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.