Objective
The present proposal deals with the involvement of a protein, BTAF1 in transcription. This process is mediated by TBP (TATA-binding protein) containing complexes. Among them B-TFIID is unique in containing only one TBP interacting protein, BTAF1 and in exhibiting ATPase activity which regulates BTAF1-TBP interaction. Little is known about this regulation.
The proposal aims i) to identify new interactors of BTAF1 and to study effects of them on BTAF1 function both in transcription and in ATPase assays and ii ) to visualize conformational changes within B-TFIID. The host laboratory is the Laboratory for physiological Chemistry managed by Marc Timmers located at the University Medical Centre of Utrecht. During my Ph.D. work I gained experience in study of protein-protein interactions.
The new stimulating environment, which represents the host laboratory, will allow me to tackle this major question from another angle. In fact, in addition to significantly improve my skills in protein expression systems, purification and protein-protein interactions assays, I will learn to master new skills as protein/DNA interactions assays and transcription assays. Thanks to collaboration with an international group I will be able to use cryo-electron microscopy to study the topology of large protein complexes. This very fruitful experience will provide me the opportunity to find an academic position in France.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics DNA
- natural sciences mathematics pure mathematics topology
- natural sciences physical sciences optics microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
UTRECHT
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.