Objective
DNA double-strand breaks (DSBs) are highly cytotoxic and mutagenic. In eukaryotes, two proteins, Mre11 and Rad50 play a major role in DSBs recombination/repair. In particular, these proteins are involved in the maturation of DSBs during homologous recombin ation which requires 3' overhangs for recombinases. However, their in vitro activities cannot explain this maturation mechanism suggesting the existence of additional partners. In thermophilic archaea homologs of Mre11 and Rad50 are organised in an operon-like structure, together with two genes encoding a nuclease and a helicase. The nuclease (NurA) exhibits 5´-3´ exonuclease activity, indicating that it could be essential for the production of the 3´overhangs (Constantinesco et al., 2002). I´m interested i n the design of an in vitro system for DSBs maturation by the Rad50-Mre11 pathway. In the proposed study I will analyse the interactions between the four proteins Mre11, Rad50, NurA and HerA and investigate the activities of the proteins as well as of the potential complexes. Subsequently I will identify additional proteins binding to the complexes using crude protein extracts from Sulfolobus and specific antibodies. In addition, I will initiate the study of in vivo functions using recently developed geneti c tools for Sulfolobus. Together this should lead to a comprehensive model of the initiation step of DNA recombination/repair in archaea.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IIF - Marie Curie actions-Incoming International Fellowships
Coordinator
ORSAY
France
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