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Human monoclonal antibodies from a library of hybridomas

Objective

Introduciton: Unlike monoclonal antibodies from hybridomas, recombinant antibodies from bacteria usually suffer from poor stability and yield. Due to surface displayed antibody molecules, recombinant antibodies are easily screened, though. (WP1) In order to combine both advantages, we plan to construct a library of >10(+6) different hybridomas by consecutive homologous and specific recombinations, with all of the hybridomas descending from one single cell. Each dell displays >100.000 of "its" specifi c antibody molecules on the cell surface. Therefore, the library should be easily screened fo low to medium affinity human antibodies specific for almost all antigens. (WP2) Antibodies of higher affinities will be generated by the apparatus responsible fo r somatic hypermutations currently being deciphered, whereby FACS-screening offers an especially easy method to compare affinities. (WP3) The ancestor cell line of our antibody library is engineered to emit signals in response to antigen binding, thus a llowing for the labelling detection of antigen-specific antibodies. Outlook: The combined efforts described above might evolve into a method that supersedes the current state of the art in the production of monoclonal antibodies.

Call for proposal

FP6-2003-NEST-PATH
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Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG
Address
Im Neuenheimer Feld 280
Heidelberg
Germany

Participants (4)

BEREICH HUMANMEDIZIN DER GEORG-AUGUST-UNIVERSITAET - STIFTUNG DES OEFFENTLICHEN RECHTS
Germany
Address
Robert-koch-str. 40
Goettingen
EUCODIS GMBH
Austria
Address
Brunner Strasse 59
Vienna
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France
Address
101 Rue De Tolbiac
Paris
INSTITUTE OF MOLECULAR GENETICS, ACADEMY OF SCIENCES OF THE CZECH REPUBLIC
Czechia
Address
Flemingovo Namesti 2
Prague 6