Skip to main content

Long-Period Observation of Single (Bio)-Molecular Motors by Minimal-Invasive Fluorescence Lifetime Imaging Nanoscopy (FLIN)

Objective

The recently invented fluorescence lifetime imaging nanoscopy (FLIN) provides a groundbreaking tool for the study of single molecules (SM) and single molecular motors (SMM) as well as a broad array of phenomena in the NanoWorld. Previous limitations for SM M studies, resolution, short observation times, and photo-dynamic reactions, are now overcome by minimal-invasive picosecond FLIN. FLIN is the extension of the extremely successful fluorescence lifetime imaging microscopy (FLIM) into the nano-domain, with 10 to 100 nm space resolution. FLIN results from the combination of 4pi-microscopy with novel ultrasensitive, nonscanning imaging detectors, based on time- and space-correlated single photon counting (TSCSPC) that allows ultra-low excitation levels. This r esults in long-period (#gt; 1 hour), minimal-invasive observation of living cells and SM/SMM, without any cell damage or irreversible bleaching. Minimal-invasive FLIN (MI-FLIN) with global point spread function modelling allows observation of SMM movement at 1-nm accuracy and 10-nm resolution. Parallel to (i) MI-FLIN/FLIM implementation, the consortium will (ii) improve sensitivity, time- and space-resolution as well as throughput of the TSCSPC detectors, (iii) explore an array of novel applications provide d by MI-FLIM/FLIN, such as nanometer SMM-tracking, (iv) develop a super-background-free TIRF microscope to improve detectability of SM/SMM, and (v) examine the behaviour of four different types of SMM and their dependence on energy-input. Enhanced basic un derstanding of biological and artificial machines and motors will lead to improved model systems and proceed one day to the design of artificial systems, improving the interface of biological and non-biological worlds. Furthermore, biological SMM are invol ved in many disease states such as Alzheimers, Werner syndrome and infectious diseases. Our studies aim to improve understanding of how these motors operate and how they break down in disease.

Call for proposal

FP6-2003-NMP-TI-3-MAIN
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

EUROPHOTON GMBH, GESELLSCHAFT FUER OPTISCHE SENSORIK
Address
Mozartstrasse 27
Berlin
Germany

Participants (9)

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
France
Address
101 Rue De Tolbiac
Paris
LEIBNIZ-INSTITUT FUER NEUROBIOLOGIE
Germany
Address
Brenneckestrasse 6
Magdeburg
PETERSBURG NUCLEAR PHYSICS INSTITUTE - RUSSIAN ACADEMY OF SCIENCE
Russia
Address
Orlowa Roscha 1
Gatchina
PHOTEK LIMITED
United Kingdom
Address
Castleham Road 26
St Leonard On Sea
ROENTDEK - HANDELS GMBH
Germany
Address
Im Vogelshaag 8
Kelkheim - Taunus
RUSSIAN RESEARCH CENTER "KURCHATOV INSTITUTE"
Russia
Address
Kurchatov Sq. 1
Moscow
THE UNIVERSITY OF EDINBURGH
United Kingdom
Address
Old College, South Bridge
Edinburgh
USTAV ORGANICKE CHEMIE A BIOCHEMIE - AKADEMIE VED CESKE REPUBLIKY
Czechia
Address
Flemingovo Nam. 2
Praha 6
VRIJE UNIVERSITEIT AMSTERDAM
Netherlands
Address
De Boelelaan 1085-1087
Amsterdam