Objective Plants can be considered as natural, solar energy powered, and environmentally friendly factories that can be improved for production of proteins with therapeutical value. However, current transgenic technologies are not accurate enough to secure reliable and precise protein production. Random chromosomal integration of transgenic inserts often causes misregulation of their transcription including complete gene silencing. Therefore the ability to modify plant genomes in a precise manner is arguably the most important missing technology in the toolkit of plant biologists. DNA integration, via homologous recombination (Gene targeting - GT) has proved a powerful technology in many species. It enables disruption, modification or replacement of an endogenous locu s by a new gene, in the native genomic context. Despite its success in other organisms, GT has not yet become a routine technique in plants. New findings, several of which from the TAGIP partners, suggest that GT became a realistic goal. To achieve this go al we propose to gain fundamental knowledge in the field of genome maintenance/modifications via DNA recombination and to apply this knowledge to develop new technologies for precise engineering of plant genomes. Finally, this knowledge will be harnessed f or the production of proteins of high value. Work will be carried in Arabidopsis and in model crop plants. The objectives of TAGIP are: -Development of new vectors and delivery methods for GT -Altering DNA recombination pathways to enhance GT -Application of GT for production of therapeutic proteins in plants The GT mechanism is a fundamental and open question. The tools and knowledge to be developed in TAGIP are relevant to agriculture (two small Agbiotech companies are active participants in the consortiu m) and medicine. The project is of particular importance for the EU states where public concern over the use of GMOs has generated a strong need for controlled integration of beneficial transgenic Fields of science natural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencesinorganic chemistrytransition metalsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsagricultural sciencesagriculture, forestry, and fisheriesagriculturenatural sciencesbiological sciencesgeneticsgenomes Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2004-1.1.0-1 - Topics for Specific Targeted Research Project in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Call for proposal FP6-2004-LIFESCIHEALTH-5 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator WEIZMANN INSTITUTE OF SCIENCE EU contribution No data Address Herzl St. 2 REHOVOT Israel See on map Links Website Opens in new window Total cost No data Participants (8) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution No data Address 2 avenue Albert Einstein VILLEURBANNE See on map Links Website Opens in new window Total cost No data UNIVERSITY OF KARLSRUHE (TH) Germany EU contribution No data Address Kaiserstr. 12 KARLSRUHE See on map Links Website Opens in new window Total cost No data INSTITUTE OF EXPERIMENTAL BOTANY AS CR Czechia EU contribution No data Address Rozvojova 135 PRAHA 6 - LYSOLAJE See on map Links Website Opens in new window Total cost No data UNIVERSITE DE GENEVE Switzerland EU contribution No data Address 24,rue du General-Dufour GENEVA 4 See on map Links Website Opens in new window Total cost No data BIOGEMMA SAS France EU contribution No data Address 1 rue Edouard Colonne PARIS See on map Links Website Opens in new window Total cost No data EVOGENE LTD Israel EU contribution No data Address 13 Gad Feinstein Street REHOVOT See on map Links Website Opens in new window Total cost No data NOVARTIS FORSCHUNGSSTIFTUNG, ZWEIGNIEDERLASSUNG FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH Switzerland EU contribution No data Address Maulbeerstr. 66 CH-4058 BASEL See on map Links Website Opens in new window Total cost No data O.S.M.-DAN LTD Israel EU contribution No data Address 38 Hanassi Harishon REHOVOT See on map Total cost No data
