Objective
Many enzymes interacting with DNA locate their target sites by facilitated diffusion or DNA translocation driven by NTP hydrolysis. The focus of attention with such enzymes in the past has been on the processes of recognition and catalysis that occurs after the target has been located. What is presently lacking and urgently needed is a better understanding of the molecular mechanics of target location and how this is coupled to recognition and catalysis. It is proposed to investigate these questions for the important processes of DNA restriction and repair. Whereas simple restriction enzymes function as homodimers, more complex ones act in concert with other enzymes and depend on ATP hydrolysis. DNA mismatch repair is yet more complicated, and several protein s, some of them ATPases, need to be recruited in the early stages of the process. We need to understand what the molecular machinery of translocation looks like, and how the process is accomplished. Given the complexity of the systems and the mechanochemical processes under study, it will be necessary to deploy a variety of approaches and advanced techniques, not available in a single institution, to the problem.
The partners in the proposed network have been recruited from academia, research institutions and SMEs and selected on the strength of their expertise in the following areas: molecular biology, bioinformatics, enzymology, analytical chemistry, and biophysics. We are confident that with this array of interdisciplinary expertise we will be well placed to make substantial progress in understanding target site location, recognition and initiation of catalysis - issues of central importance for all enzymes acting on DNA. A thorough understanding of these systems will certainly enable better use to be made of them. We believe that young researchers trained in our network will greatly benefit from our integrated intersectorial approach and will be well placed for contemporary academic and industrial research.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences analytical chemistry
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences catalysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences molecular biology structural biology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-1
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
GIESSEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.