Objective Many enzymes interacting with DNA locate their target sites by facilitated diffusion or DNA translocation driven by NTP hydrolysis. The focus of attention with such enzymes in the past has been on the processes of recognition and catalysis that occurs after the target has been located. What is presently lacking and urgently needed is a better understanding of the molecular mechanics of target location and how this is coupled to recognition and catalysis. It is proposed to investigate these questions for the important processes of DNA restriction and repair. Whereas simple restriction enzymes function as homodimers, more complex ones act in concert with other enzymes and depend on ATP hydrolysis. DNA mismatch repair is yet more complicated, and several protein s, some of them ATPases, need to be recruited in the early stages of the process. We need to understand what the molecular machinery of translocation looks like, and how the process is accomplished. Given the complexity of the systems and the mechanochemical processes under study, it will be necessary to deploy a variety of approaches and advanced techniques, not available in a single institution, to the problem.The partners in the proposed network have been recruited from academia, research institutions and SMEs and selected on the strength of their expertise in the following areas: molecular biology, bioinformatics, enzymology, analytical chemistry, and biophysics. We are confident that with this array of interdisciplinary expertise we will be well placed to make substantial progress in understanding target site location, recognition and initiation of catalysis - issues of central importance for all enzymes acting on DNA. A thorough understanding of these systems will certainly enable better use to be made of them. We believe that young researchers trained in our network will greatly benefit from our integrated intersectorial approach and will be well placed for contemporary academic and industrial research. Fields of science natural scienceschemical sciencesanalytical chemistrynatural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencescatalysisnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesnatural sciencesbiological sciencesmolecular biologystructural biology Keywords Catalysis Crosslinking DNA Enzymes DNA Recognition DNA Translocation Mass Spectrometry Protein-DNA and Protein-Protein-Interactions Single Molecule Fluorescence Spectroscopy Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Topic(s) MOBILITY-1.1 - Marie Curie Research Training Networks (RTN) Call for proposal FP6-2004-MOBILITY-1 See other projects for this call Funding Scheme RTN - Marie Curie actions-Research Training Networks Coordinator JUSTUS-LIEBIG-UNIVERSITÄT EU contribution No data Address Ludwigstrasse 23 GIESSEN Germany See on map Links Website Opens in new window Total cost No data Participants (11) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITY OF BRISTOL United Kingdom EU contribution No data Address Senate House, Tyndall Avenue BRISTOL See on map Links Website Opens in new window Total cost No data INSTITUTE OF ENZYMOLOGY - BIOLOGICAL RESEARCH CENTER - HUNGARIAN ACADEMY OF SCIENCES Hungary EU contribution No data Address Karolina ut 29 BUDAPEST See on map Links Website Opens in new window Total cost No data UNIVERSITY OF NEWCASTLE UPON TYNE United Kingdom EU contribution No data Address 6 Kensington Terrace NEWCASTLE UPON TYNE See on map Links Website Opens in new window Total cost No data UNIVERSITY OF SOUTHERN DENMARK Denmark EU contribution No data Address Campusvej 55 ODENSE M See on map Links Website Opens in new window Total cost No data INSTITUTE OF BIOTECHNOLOGY Lithuania EU contribution No data Address V. Graiciuno 8 VILNIUS See on map Links Website Opens in new window Total cost No data FERMENTAS UAB Lithuania EU contribution No data Address V. Graiciuno 8 VILNIUS See on map Links Website Opens in new window Total cost No data INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY Poland EU contribution No data Address Ks. Trojdena 4 WARSAW See on map Links Website Opens in new window Total cost No data UNIVERSITY OF YORK United Kingdom EU contribution No data Address Heslington YORK See on map Links Website Opens in new window Total cost No data REPLIZYME LIMITED United Kingdom EU contribution No data Address Genesis 2 Building, York Science Park HESLINGTON, YORK See on map Links Website Opens in new window Total cost No data CHARITE UNIVERSITÄTSMEDIZIN BERLIN Germany EU contribution No data Address Schumannstrasse 20-21 BERLIN See on map Links Website Opens in new window Total cost No data HEINRICH-HEINE-UNIVERSITÄT DÜSSELDORF Germany EU contribution No data Address Universitätsstrasse 1 DÜSSELDORF See on map Links Website Opens in new window Total cost No data