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Enhanced sensitivity Nanotechnology-based Multiplexed Bioassay Platform for diagnostic applications

Objective

Currently, there is strong interest in the development of new bioassay techniques for gene identification, gene mapping, DNA sequencing and medical diagnostics. There are three main families of methods: Polymerase Chain Reaction, Enzyme-Linked Immunosorbent Assay and nano-particles agglutination techniques. All these methods suffer from several disadvantages as they are time-consuming and expensive, they are not quantitative and exclude multiplexing, i.e. the detection of different genotypes simultaneously. The need of a new multiplexing and quantitative bioassay technique is evident. The aim of this project is to develop a high sensitivity multiplexed platform based on a bio-non bio nanostructure able to enhance diagnostic capabilities by exploiting the dimensional shift from bio-systems to nanometric particles, thus overcoming many of the limitations of the existing methods. This method could be adapted to the detection of many kinds of bio-systems, but the project will focus on Human Papilloma Virus (HPV) responsible for cancer. The project idea is based on the development of nanoparticles functionalised with probes complementary to HPV DNA conservative region and an array of specific bio-probes for the different HPV genotypes deposited on a solid substrate. The nanoparticles will bind to the bio-system and then they will diffuse through the suspension docking to the area of the array where the probe specific for that genotype is coated. An array of nanoparticles will be created and the concentration of each HPV genotype can be quantified by estimating the number of particles bounded to each specific area. Considering the global worldwide market of the immune and genetic tests (20 Billion €) the potential economic impact can be up to 100 M€. Private/public national or local health service providers will get benefits from NANO-MUBIOP, the single test cost being about 4 € for the service provider. Last but not least, the costs will be reduced for the patients.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/dna

Call for proposal

FP7-NMP-2007-SMALL-1
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

HOSPITEX DIAGNOSTICS SRL
Address
Via Provinciale Lucchese 145
50019 Sesto Fiorentino
Italy
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
EU contribution
€ 775 800
Administrative Contact
Stefania Fammoni (Dr.)

Participants (7)

INSTITUT PASTEUR
France
EU contribution
€ 204 745
Address
Rue Du Docteur Roux 25-28
75724 Paris Cedex 15
Activity type
Research Organisations
Administrative Contact
Nadia Khelef (Dr.)
DUBLIN CITY UNIVERSITY
Ireland
EU contribution
€ 245 875
Address
Glasnevin
9 Dublin
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Eugene Kennedy (Prof.)
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
France
EU contribution
€ 146 575
Address
147 Rue De L'universite
75007 Paris Cedex 07
Activity type
Research Organisations
Administrative Contact
Angelo Trussardi (Mr.)
XENNIA TECHNOLOGY LIMITED

Participation ended

United Kingdom
EU contribution
€ 177 615
Address
Works Road Monroe House
SG6 1LN Letchworth
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Nicholas Balon (Mr.)
URATIM GYARTO KORLATOLT FELELOSEGU TARSASAG
Hungary
EU contribution
€ 176 840
Address
Ugron Gábor Utca 8
4400 Budapest
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Vince Grolmusz (Prof.)
LABORATORIO EUROPEO DI SPETTROSCOPIE NON LINEARI
Italy
EU contribution
€ 387 700
Address
Via Nello Carrara 1
50019 Sesto-fiorentino (Fi)
Activity type
Research Organisations
Administrative Contact
Cristina Cipullo (Ms.)
UNIVERSITA DEGLI STUDI DI FIRENZE
Italy
EU contribution
€ 397 000
Address
Piazza San Marco 4
50121 Florence
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Gianna Giusti (Dr.)