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Role of Poplar RBOH family protein in ozone-induced oxidative stress: assessment of the protein activity regulation in planta and development of biochemical tools for analysis of the protein funtion

Objective

The increase in tropospheric ozone concentrations has been considered to be one of the major reasons for yield reductions in many crop plants and thus to economically significant losses. Natural environments, and forest stands especially, have received as hare of this pollutant insult.

Evidence from previous research indicates that ozone-induced plant responses may be mechanistically similar to pathogen-induced plant responses such as the hypersensitive response. It has been shown by combining localization, enzyme activity and inhibitor studies that the plasma membrane NADPH oxidase, a respiratory burst oxidase homologue (RBOH), is one of most likely candidates for stress-induced reactive oxygen species production in trees.

This project will aim to evaluate a role of different RBOH proteins in O3-induced oxidative stress in poplar trees, to develop biochemical tools for analysis of structural aspects of activity regulation of the RBOH enzymes involved in hypersensitive response of plants and to investigate a potential of the NADPH, a RBOH enzyme substrate, biosynthesis pathway modification to modulate ROS production and possibly to enhance the resistance of trees to environmental pollution.

Call for proposal

FP6-2004-MOBILITY-12
See other projects for this call

Funding Scheme

IRG - Marie Curie actions-International re-integration grants

Coordinator

LITHUANIAN FOREST RESEARCH INSTITUTE
Address
Liepu 1
Girionys, Kaunas Distr.
Lithuania