The increase in tropospheric ozone concentrations has been considered to be one of the major reasons for yield reductions in many crop plants and thus to economically significant losses. Natural environments, and forest stands especially, have received as hare of this pollutant insult.
Evidence from previous research indicates that ozone-induced plant responses may be mechanistically similar to pathogen-induced plant responses such as the hypersensitive response. It has been shown by combining localization, enzyme activity and inhibitor studies that the plasma membrane NADPH oxidase, a respiratory burst oxidase homologue (RBOH), is one of most likely candidates for stress-induced reactive oxygen species production in trees.
This project will aim to evaluate a role of different RBOH proteins in O3-induced oxidative stress in poplar trees, to develop biochemical tools for analysis of structural aspects of activity regulation of the RBOH enzymes involved in hypersensitive response of plants and to investigate a potential of the NADPH, a RBOH enzyme substrate, biosynthesis pathway modification to modulate ROS production and possibly to enhance the resistance of trees to environmental pollution.
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