Objective
Cell based therapies potentially allow for alternative treatment of several human diseases. The search for an autologous cell source merits the investigation of novel means to de-program patient derived differentiated cells into pluripotent embryonic stem (ES) cells (or ES-like cells) with therapeutic potentials. The technique of cloning by somatic cell nuclear transfer (SCNT), where a differentiated somatic cell is transferred into an enucleated oocyte has now in several mammalian species proven that the oocyte possesses factors that can entirely de- and re-program the somatic cell genome.
In this project, cytoplasmic extract of bovine oocytes obtained at the slaughterhouse will be used to de-program bovine and human granulosa cells into potential pluripotent ES-like cells. Recent data from the host laboratories indicate that the nucleolus can be used as a marker for genomic de-programming in conjunction with SCNT and that the de-programming effect of the oocyte is not species specific. Building on this back ground, we intend to use the nuclear and nucleolar architecture as well as markers of pluripotency of a somatic cell exposed to extracts from oocyte cytoplasm, to examine whether de-programming of the somatic cell genome is possible and to gain insight into the underlying molecular changes that are required to induce gene de-programming. This new knowledge generated in this research training project will constitute the basis for creating ES or ES-like cells for autologous cell replacement therapy circumventing the need for producing SCNT-embryos for ES cell derivation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences medical biotechnology cells technologies stem cells
- natural sciences biological sciences genetics RNA
- medical and health sciences clinical medicine embryology
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
COPENHAGEN K
Denmark
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.