Objective
Alternative pre-mRNA splicing plays a central role in generating the complex proteome of metazoans. About 60% of human genes are alternatively spliced and a compromise in this process is known to cause multiple human diseases. An understanding of the mecha nisms that regulate alternative splicing will be essential for a functional interpretation of the genome. We propose to study the co-transcriptional recruitment of splicing regulatory proteins-specifically SR proteins- onto the pre-mRNA. Such an analysis w ould be valuable since the coupling of splicing to transcription has been shown to contribute to alternative splicing regulation. My approach will involve analysis of RNA while it is still attached to the chromosomal DNA, during the act of transcription by RNA polymeraseII. Recruitment of the basic splicing machinery to target genes by this novel method has been shown in the host lab. I propose to examine the detectability and distribution of SR proteins on a candidate gene that undergoes alternative splici ng to encode either calcitonin or the neuropeptide CGRP. Through chromosomal immunoprecipitation (CHIP) I will determine the sites along the gene at which the SR proteins are recruited. Since the splicing of this gene can be manipulated in cell culture, I will be able to determine the consequential changes in SR protein recruitment. This will be followed by analysis of changes in recruitment pattern when critical sequences in the gene are altered. In a complementary approach we will alter the levels of the SR protein and analyse its affect on alternative splicing regulation. Lastly, I will extend the analysis to a global scale by carrying out CHIP followed by hybridization to DNA microarrays, designed to detect genomic intron and exon regions. This study wil l identify the yet unknown endogenouos gene targets for the SR proteins. Thus, I will be able to build a comprehensive picture of splicing factor recruitment and its regulation of alternative splicing.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-7
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IIF - Marie Curie actions-Incoming International Fellowships
Coordinator
DRESDEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.