Objective
G protein-coupled receptor kinases (GRK) provide the rate-limiting step for terminating incoming signals at G protein-coupled receptors (GPCR) to protect the cell from over-stimulation. GRK2, the most commonly expressed GRK, has been shown to regulate hear t function in the adult and during development as GRK2 knockout (KO) mice die during embryonic development. Since the reason for the embryonic lethality has been analysed only preliminary, I want to uncover the molecular mechanism to provide a biochemical explanation for the action of GRK2 in ensuring normal cardiac development and in the course of cardiovascular diseases.
The aims of the project are:
1) Generation of a GRK2 knockdown in zebra fish and its histological and biochemical characterization.
2) De termination of the GRK2 structures relevant for the assessed phenotype.
3) Investigation of affected signalling cascades.
4) Transfer of the zebrafish results back to mice.
The novelty of the project is based on a combined in vivo approach to explain the bio chemical function of a protein of highest relevance in man by using a second model organism instead of extrapolating in vivo relevance from in vitro or cell culture studies. To accomplish this, I will be trained in manipulating and analysing zebra fish and in techniques commonly used in signal transduction such as site-directed cloning and biochemical assays, which will complement my previous research experience in mice and chicken embryos. In addition, I will not only widen my international experience in research, but also my personal horizon.
Therefore, the Marie Curie Out-going International Fellowship will serve my professional growth by enabling me to gain knowledge in the investigation of signal transduction and the handling of a third model organism, giving me the possibility to choose the technique and the organism whichever is best for the question I want to address in my future independent research career in Europe.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- medical and health sciences clinical medicine cardiology cardiovascular diseases
- natural sciences chemical sciences analytical chemistry mass spectrometry
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-6
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
OIF - Marie Curie actions-Outgoing International Fellowships
Coordinator
79095 FREIBURG IM BREISGAU
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.