RNA-based silencing mechanisms were first recognised from descriptions of co-suppression in transgenic plants and of RNA interference with double stranded RNA in C. elegans. Recently it had become apparent that both experimental silencing mechanisms recapi tulate a natural evolutionary conserved RNA-mediated silencing mechanism. This mechanism is present in all phyla of eukaryotes and can be placed in at least three pathways: 1) cytoplasmic short interfering (si)RNA silencing, 2) micro (mi)RNA silencing, and 3) nuclear siRNA silencing. Argonaute (AGO) proteins have been implicated with all three RNA silencing pathways in plants, animals and fungi. It seems likely that AGO proteins are integral components of the RNA silencing mechanism. In this proposal it is proposed to further investigate silencing pathways by focussing on the AGO proteins in Arabidopsis.
Field of science
- /natural sciences/biological sciences/genetics and heredity/rna
- /natural sciences/biological sciences/biochemistry/biomolecules/proteins
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