Objective
Traces of surviving degraded DNA in marginal clinical, forensic, archaeological, and environmental samples are generally amplified by PCR, which can often produce highly-skewed product distributions dominated by non-authentic sequences (due to DNA damage and PCR-generated artefacts). The applicant has developed the SPEX amplification methodology, an alternative to PCR which has generated sequence data of unprecedented accuracy from specimens up to 60,000 years old. SPEX creates first-generation copies from target template strands at defined loci and can generate a quantifiable redundancy of coverage (providing in-built controls for the detection of contamination and allelic drop-out). SPEX has provided key insights and solved long-standing puzzles in the molecular processes of DNA degradation. The applicant would be fully-trained in every aspect of state-of-the-art multiplexed genotyping methodologies, at a host institution currently providing high-throughput data for population genetics, disease association and forensics studies in worldwide collaborations. The proposed project to develop multiplexed-SPEX systems could reduce genotyping errors down towards the background level of polymerase misincorporation, allowing the rapid and reliable quantifiable genotyping of even highly marginal samples. This could revolutionise the authentication of sequence data in areas as diverse as basic cancer research, forensics, biomolecular archaeology, and conservation biology. In addition, highly-promising prototypes for the whole genome amplification of marginal DNA could allow key samples to be 'immortalised' for prolonged in-depth genetic analyses. The proposed project would create new hybrid technologies, useful in many fields throughout the EU and beyond. The enthusiastic backing of international collaborators provides scope for EU-wide technology transfer, and would leave the applicant with the core skills to take these novel methodologies into further research positions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences clinical medicine oncology
- humanities history and archaeology archaeology
- natural sciences biological sciences genetics genomes
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
28015 Madrid
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.