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Directed Evolution in Femtolitre Microreactors - Exploring 'Promiscuity Space'

Objective

The ‘vastness of sequence space’, i.e. the extremely large number of possible permutations in even the smallest catalytic proteins, requires evolutionary shortcuts. In this project the candidate will interconvert members of a promiscuous catalytic superfamily into one anotehr and differentiate a multi-specific enzyme into mono-specific mutants. Ultimately it is hoped that directed evolution experiments will have implications for the definition of ‘phylogenetic relationships’ for catalysis. To this end the candidate will set up biochemical assays for hydrolytic reactions in small emulsion droplets (diameter: 2-20 um) and explore whether (i) these assays can be carried out in such in vitro compartemnts , (ii) whether it is possible to measure kinetics in these droplets oand (iii) carry out directed evolutino experiments with a sreening system of unprecedented capacity. In addition to tehse technical aspects, the candidate will also charcterise the mechanism of the wild type enzyme and its mutants.

Field of science

  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

FP7-PEOPLE-2007-2-1-IEF
See other projects for this call

Funding Scheme

MC-IEF - Intra-European Fellowships (IEF)
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Coordinator

THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE
Address
Trinity Lane The Old Schools
CB2 1TN Cambridge
United Kingdom
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 177 173,03
Administrative Contact
Wendy Holman (Ms.)