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Novel high-performance enzymes and micro-organisms for conversion of lignocellulosic biomass to bioethanol

Objective

The NEMO project provides novel efficient enzymes and microbes for 2nd generation bioethanol production. It generates through metabolic engineering and mutagenesis & screening approaches robust yeast strains that have a broad substrate range and can (co-)ferment C6 and C5 sugars to ethanol with high productivity (rate and yield), and that are significantly more stress tolerant, i.e. inhibitor, ethanol and thermotolerant than the current S.cerevisiae strains used in ethanol production. The NEMO project also identifies and improves enzymes for hydrolysis of biomasses relevant for Europe. Novel enzymes are identified and improved through various approaches, based on screening, broad comparative genomics analyses, and protein engineering. These efforts will generate more thermostable enzymes for high temperature hydrolysis, more efficient enzymes for hydrolysis of the resistant structures in lignocellulose such as crystalline cellulose and lignin-hemicellulose complexes, enzymes with reduced affinity on lignin, and efficient thermo and mesophilic enzyme mixtures that are optimised and tailor-made for the relevant biomasses for Europe and European industry. These novel biocatalysts are tested in an iterative manner in process relevant conditions, including also pilot-scale operations, which ensure that the novel enzymes and microbes will be superior in real process conditions. Furthermore, optimal enzyme, microbe and process regime combinations are identified, providing basis for the development of the most economic and ecoefficient overall processes. The impact of the NEMO project on 2nd generation bioethanol production is significant because it provides most realistic but widely applicable technologies that could be exploited broadly by European industry. Its impact goes also much beyond bioethanol because NEMO provides technology improvements that are directly applicable and crucial for efficient and economic production of also other biofuels and bulk chemicals.

Field of science

  • /engineering and technology/environmental engineering/energy and fuels/biofuels
  • /agricultural sciences/agricultural biotechnology/biomass
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes
  • /engineering and technology/industrial biotechnology/metabolic engineering
  • /social sciences/economics and business/economics/production economics/productivity

Call for proposal

FP7-KBBE-2007-2A
See other projects for this call

Funding Scheme

CP-IP - Large-scale integrating project

Coordinator

TEKNOLOGIAN TUTKIMUSKESKUS VTT
Address
Tekniikantie 4 A
02044 VTT Espoo
Finland
Activity type
Research Organisations
EU contribution
€ 1 202 075
Administrative Contact
Seija Flinck (Ms.)

Participants (20)

LUNDS UNIVERSITET
Sweden
EU contribution
€ 579 802
Address
Paradisgatan 5C
22100 Lund
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Marie Gorwa-Grauslund (Dr.)
HELSINGIN YLIOPISTO
Finland
EU contribution
€ 312 848
Address
Yliopistonkatu 3
00014 Helsingin Yliopisto
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Sanna-Maija Miettinen (Dr.)
UNIVERSITEIT UTRECHT
Netherlands
EU contribution
€ 409 901
Address
Heidelberglaan 8
3584 CS Utrecht
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Marcel De Wit (Mr.)
VIB VZW
Belgium
EU contribution
€ 456 500
Address
Rijvisschestraat 120
9052 Zwijnaarde - Gent
Activity type
Research Organisations
Administrative Contact
Rik Audenaert (Mr.)
CHALMERS TEKNISKA HOEGSKOLA AB
Sweden
EU contribution
€ 407 688
Address
-
41296 Goeteborg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Karin Westerlund (Ms.)
JOHANN WOLFGANG GOETHE-UNIVERSITATFRANKFURT AM MAIN
Germany
EU contribution
€ 355 800
Address
Theodor W Adorno Platz 1
60323 Frankfurt Am Main
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Christoph Denecke (Mr.)
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Switzerland
EU contribution
€ 208 875
Address
Batiment Ce 3316 Station 1
1015 Lausanne
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Vassily Hatzimanikatis (Prof.)
UNIVERSITE D'AIX MARSEILLE
France
EU contribution
€ 1 981,34
Address
Boulevard Charles Livon 58
13284 Marseille
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Céline Damon (Ms.)
UNIVERSITA' DEGLI STUDI DI MILANO-BICOCCA
Italy
EU contribution
€ 258 768
Address
Piazza Dell'ateneo Nuovo 1
20126 Milano
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Anastasia Sguera (Dr.)
KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW
Netherlands
EU contribution
€ 157 500
Address
Kloveniersburgwal 29 Het Trippenhuis
1011 JV Amsterdam
Activity type
Other
Administrative Contact
Don Van Velzen (Mr.)
UNIVERZA V LJUBLJANI
Slovenia
EU contribution
€ 75 000
Address
Kongresni Trg 12
1000 Ljubljana
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Maja Bucar (Ms.)
CHEMTEX ITALIA SRL
Italy
EU contribution
€ 214 600
Address
Strada Ribrocca 11
15057 Tortona Alessandria
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Alessandra Frattini (Dr.)
DYADIC NEDERLAND BV
Netherlands
EU contribution
€ 363 435
Address
Nieuwe Kanaal 7 S
6709 PA Wageningen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Willem Van Der Wilden (Dr.)
GREEN SUGAR GMBH PRODUKTINNOVATIONEN AUS BIOMASSE
Germany
EU contribution
€ 277 950
Address
Hermann Grafe Strasse 40
01662 Meissen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Frank Kose (Mr.)
SEKAB E-TECHNOLOGY AB
Sweden
EU contribution
€ 249 997
Address
Hoerneborgsvaegen
89121 Oernskoeldsvik
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Tina Vedin (Ms.)
ROAL OY
Finland
EU contribution
€ 100 219
Address
Tykkimaentie 15
05200 Rajamaki
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Heikki Sakari Halonen (Mr.)
C-LECTA GMBH
Germany
EU contribution
€ 135 120
Address
Perlickstrasse 5
04103 Leipzig
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Carsten Fietz (Mr.)
SYNGENTA CROP PROTECTION AG

Participation ended

Switzerland
EU contribution
€ 1 615
Address
Schwarzwaldallee 215
4058 Basel
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Emanuele Pizzo (Mr.)
WIRTSCHAFT UND INFRASTRUKTUR GMBH & CO PLANUNGS KG
Germany
EU contribution
€ 54 707
Address
Sylvensteinstrasse 2
81369 Muenchen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Christine Meyer-Haege (Ms.)
UNIVERSITE DE LA MEDITERRANEE D'AIX-MARSEILLE II

Participation ended

France
EU contribution
€ 73 618,66
Address
Boulevard Charles Livon, Jardin Du Pharo 58
13284 Marseille
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Céline Damon (Ms.)