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High-Density Peptide MicroArrays and Parallel On-line Detection of Peptide-Ligand Interactions

Objective

"Proteins are crucial structural and functional elements of life, and frequent targets of intervention. Short protein sequences, conventionally defined as peptides, have emerged as indispensable tools in biotechnology: peptides may represent short linear protein structures, and in some cases they even mimic more complex discontinuous structures. Custom-made synthetic peptides cost down to ca. 30 EURO (decamer, few mg, moderate purity); yet, a comprehensive screening program can easily cost 100,000'es of EUROs, and the cost of the screening program may itself be prohibitive due to the logistics of assaying many individual peptides. Thus, the pharmaceutical and biotechnology industries, as well as academia, encounter significant economic and logistical barriers, when they attempt to apply peptide-based screening programmes in the exploitation of ""-omics"" information. In the current proposal, we will use novel photochemistry to synthesise large arrays of peptides on chips the size of a stamp (from a few thousand up to more than a hundred thousand defined peptides) reducing the cost of synthesis to a few cents per peptide. To detect analyte interactions with individual peptide moieties, we use both label-dependent optical detection (10e4 to 10e5 different peptides per chip), as well as label-independent recording of binding kinetics measuring rates and strengths of interactions simultaneously for up to 2000 peptides. Bioinformatics will be used to interpret microarray data, and guide the design of subsequent iterative peptide microarrays thereby greatly enhancing the number of peptide specificities that otherwise can be handled at the level of a single peptide chip. This will allow acquisition of peptide and protein data at an unprecedented scale and quality, at a fraction of the cost and time associated with other current technologies. For the SME's, this project will support their innovation and validation, and provide significant new market possibilities."

Field of science

  • /social sciences/economics and business/business and management/commerce
  • /natural sciences/chemical sciences/physical chemistry/photochemistry
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins

Call for proposal

FP7-HEALTH-2007-B
See other projects for this call

Funding Scheme

CP-TP - Collaborative Project targeted to a special group (such as SMEs)

Coordinator

KOBENHAVNS UNIVERSITET
Address
Norregade 10
1165 Kobenhavn
Denmark
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 470 010
Administrative Contact
Ivan Kristoffersen (Mr.)

Participants (6)

SCHAFER -N APS
Denmark
EU contribution
€ 747 667
Address
Kystvej 6
3050 Humlebaek
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Claus Nielsen (Dr.)
GENOPTICS SA
France
EU contribution
€ 589 454
Address
Plateau Du Moulon 503
91400 Orsay
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Emmanuel Maillart (Dr.)
JRC -JOINT RESEARCH CENTRE- EUROPEAN COMMISSION
Belgium
EU contribution
€ 258 024
Address
Rue De La Loi 200
1049 Brussels
Activity type
Research Organisations
Administrative Contact
Eric Cremer (Mr.)
DANMARKS TEKNISKE UNIVERSITET
Denmark
EU contribution
€ 254 854
Address
Anker Engelundsvej 1 Bygning 101 A
2800 Kgs Lyngby
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ernst Loewe Lindberg (Mr.)
KUNGLIGA TEKNISKA HOEGSKOLAN
Sweden
EU contribution
€ 319 680
Address
Brinellvagen 8
100 44 Stockholm
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Aake Eriksson (Dr.)
UNIVERSITAETSMEDIZIN DER JOHANNES GUTENBERG-UNIVERSITAET MAINZ
Germany
EU contribution
€ 299 376
Address
Langenbeckstrasse 1
55131 Mainz
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Silvia Tschauder (Dr.)