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Content archived on 2024-05-29

Improving the detection sensitivity for protein micro-arrays: wave-guide SPR imaging and nano-particle enhanced imaging

Objective

Surface plasmon resonance (SPR) spectroscopy has proven its utility and versatility in many studies, with a focal point in biochemical investigations. SPR is regarded as the most sensitive method in label-free analysis. Surface plasmon resonance imaging (S PRi) improves on the single spot interrogation of a sample by imaging an area of the sample, which can consist of a diverse sample matrix. Instead of monitoring the angular shift of the reflection minimum, a fixed angle measurement near the SPR angle is performed.

A very high detection sensitivity comparable to that of fluorescence spectroscopy is often demanded in various measurements to probe low concentration binding/interactions or weak affinities. Research efforts will focus on improvement of the detect ion sensitivity by developing wave-guide SPR imaging (WSPRi) and using nanoparticles for signal enhancement. The use of WSPRi allows for the excitation of plasmon resonance with both p and s polarized light. The resulting reflectivity spectra posses a much reduced half-width which translates to a higher sensitivity in imaging mode. Nanoparticle functionalised antibodies in combination with silver staining will be applied and the protocol optimised, aiming at a signal improvement by two to three orders of magnitude compared to existing SPR imaging assays.

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Topic(s)

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Call for proposal

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FP6-2004-MOBILITY-7
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Funding Scheme

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IIF - Marie Curie actions-Incoming International Fellowships

Coordinator

QUEEN MARY & WESTFIELD COLLEGE, UNIVERSITY OF LONDON,
EU contribution
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