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Content archived on 2024-05-29

A new approach for genic SNP discovery in polyploid bread wheat

Objective

Single nucleotide polymorphisms have become the 'industry standard' marker type in mammalian and human genetics, and will be important for a biotechnology-oriented approach to genotype improvement and redesign. The project aims to develop a generic platform for genic SNP discovery in the major temperate cereal crop species, bread wheat. Polyploidy compromises the standard approach for sequence variation detection in specific amplicons, and to address this, a strategy using SSCP separation of complex amplicons to develop homoeologue-specific PCR assays is proposed. The proposal will exploit the rice genome sequence and the existence of synteny among the grass genomes to drive primer design, and this is complemented with a SNP discovery platform based on heteroduplex identification using dHPLC technology.

Fields of science (EuroSciVoc)

Keywords

Topic(s)

Call for proposal

FP6-2002-MOBILITY-5
See other projects for this call

Funding Scheme

EIF - Marie Curie actions-Intra-European Fellowships

Coordinator

JOHN INNES CENTRE
EU contribution
No data
Address


United Kingdom
Total cost
No data