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Exploiting the saccharification potential of pathogenic microorganisms to improve biofuel production from plants

Objective

"FUEL-PATH aims at providing new knowledge on plant cell wall and innovative biotechnological solutions for biomass utilization. A key process for biomass utilization is the initial degradation of cell walls into fermentable sugars (saccharification); this is hindered by the wall recalcitrance to hydrolysis. We propose to improve the plant saccharification characteristics by mimicking a strategy successfully used by phytopathogenic microorganisms. These produce pectic enzymes before other cell wall-degrading enzymes (CWDEs) to weaken the linkages between the wall components and favour the maceration of the plant tissue. Homogalacturonan (HGA), a major component of pectin, is synthesized in a methylated form and is de-esterified in the wall by methylesterases (PMEs). De-esterified HGA interacts with calcium to form ""egg-box"" structures, which are critical for maintaining the integrity of the entire wall. We propose to improve saccharification by expression in plants of microbial polygalacturonases (PGs) hydrolizing HGA. Plants expressing a fungal PG have reduced levels of HGA and enhanced saccharification (unpublished preliminary data). Since PG activity in pianta affects normal growth, a technology of enzyme control through the use of specific protein inhibitors will be developed. A second strategy to be adopted for weakening the ""egg-box"" is the overexpression of PME inhibitors. This may cause not only an increased degradability but also an enhanced biomass production. FUEL-PATH will provide detailed information on the structure, function and construction of tailor-made enzymes and inhibitors suitable for the saccharification process. FUEL-PATH will also address the relationship between pectin composition and developmental responses mediated by hormones in PG-expressing plants. A genetic screen will be performed to isolate genes involved growth defects and increased cell wall degradability and these will be characterized for a possible biotechnological use."

Field of science

  • /natural sciences/biological sciences/microbiology
  • /agricultural sciences/agricultural biotechnology/biomass
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

ERC-2008-AdG
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Funding Scheme

ERC-AG - ERC Advanced Grant

Host institution

UNIVERSITA DEGLI STUDI DI ROMA LA SAPIENZA
Address
Piazzale Aldo Moro 5
00185 Roma
Italy
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 2 099 600
Principal investigator
Felice Cervone (Prof.)
Administrative Contact
Simone Ferrari (Dr.)

Beneficiaries (1)

UNIVERSITA DEGLI STUDI DI ROMA LA SAPIENZA
Italy
EU contribution
€ 2 099 600
Address
Piazzale Aldo Moro 5
00185 Roma
Activity type
Higher or Secondary Education Establishments
Principal investigator
Felice Cervone (Prof.)
Administrative Contact
Simone Ferrari (Dr.)