Objective
In order to understand how the mammalian immune system provides protection against pathogens, it is important to understand the molecular basis of immune system function. CD244 is one of the receptors related to CD150 (SLAM) with the potential to bind the adaptors, SAP and EAT-2. It has recently been shown by the host laboratory that the outcome of engagement of these receptors depends on the context of the interactions, such as which other receptors are engaged, the level of activation, and between which cells. The overall goal of this project will be to establish a paradigm for the function of the family of CD150-related receptors. With an initial analysis of CD2 and CD244, and later inclusion of additional family members, a thorough understanding of how this family of receptors modulates leukocyte function will be developed. Our specific aims are as follows. First, we plan to explore the parameters which determine the overall effect of CD244 and CD2 engagement during an immune response. Using a human NK cell line expressing CD2 and CD244, and a mouse T-cell hybridoma, we will examine the effects of the engagement of these receptors and recruitment of SAP and EAT-2 on cytotoxicity and IFN-gamma production. Second we will perform in vivo experiments relating human data on CD244 function to a mouse tumour model, in which the in vivo activity of a CD244 blocking antibody will be used to analyze the effect of CD244 blockade in resistance to tumour growth. Finally, using surface plasmon resonance and recombinant proteins, we will establish a hierarchy of likely interactions of intracellular signalling molecules with additional members of the CD150 receptor family. Using the model system established in the first two aims, the predictions derived from the BIAcore experiments can be tested for functional accuracy, and a greater paradigm for the function of this receptor family can be established.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences condensed matter physics quasiparticles
- natural sciences biological sciences cell biology cell signaling
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine immunology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IIF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
OX1 2JD Oxford
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.