Objective
MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression of complementary mRNAs. They have diverse unique expression patterns and have been implicate in a number of biological processes. It has been shown that in many physiological and pathological conditions individual miRNAs are subjected to post-transcriptional regulation, both at the level of Drosha and/or Dicer processing. The host lab has recently found that hnRNP A1, a protein implicated in many aspects of RNA processing, act as an auxiliary factor for the Drosha-mediated processing of a microRNA precursor, pri-miR-18a. hnRNP A1 binds to the loop of this pri-miRNA and induces a relaxation at the stem creating a more favourable cleavage site for Drosha. Our first objective is to define the functional domains in hnRNP A1 that are required for miR-18a processing. Thus, we will determine whether RNA binding and/or the unwinding activities of hnRNP A1 are required for miR-18a processing. The host lab also found that approximately 14% of all pri-miRNAs have highly conserved loops suggesting that these conserved loops act as landing pads for trans-acting factors influencing miRNA processing. In order to address the question of how widespread is the contribution of hnRNP A1 and other auxiliary factors to pri-miRNA processing I propose to identify other microRNAs that require hnRNP A1 for their processing. Secondly, I plan to identify different auxiliary factors required for the processing of microRNAs containing a conserved terminal loop by affinity pull-down assays followed by peptide mass fingerprint analysis. In summary, experiments carried out in this proposal will reveal the level of complexity for the regulation of microRNA biogenesis and their contribution to physiological states and pathological conditions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
W1B 1AL LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.