Objective
Iron is an essential nutrient. It is involved in a great variety of biological processes, such as photosynthesis or the oxidative respiration. Iron deficiency is a factor limiting crop production, and the most important dietary deficiency in humans. In order to address these problems, plant iron uptake has been studied with detail. As a result, metal transporters of the ZIP family have been identified as the main responsibles for iron uptake from soil in plants, with the exception of some grasses. However, very little is known on their biochemistry and on the shuttling of iron in the cytosol. The aim of this proposal is to biochemically characterized these ZIP transporters, using as a model Arabidopsis thaliana IRT1. This transporter has successfully been expressed in yeast, where it is still active and therefore is susceptible of being purified in an active form. Oligomeric state of the protein, metal specificity and kinetical parameters will be determined in vitro, by means of fluorescent probes specific for the different transport substrates. Similarly, the possibility of cotransport will be evaluated. Metal affinity, metal binding site/-s will be determined by the use of fluorescent probes and site directed mutagenesis, and further verified with X-ray spectroscopy. The acceptor from the iron introduced by IRT1 will be identified by means of copurification, coimmunoprecipitation or by chemical crosslinking. All these results will be further validated in vivo in an Arabidopsis irt1-1 background. As a result of this work we will determine how IRT1 works, the first time that this would be done in a ZIP transporter. These results will be used in the characterization of other ZIP transporters regardless their origin, plant, animal, bacteria ,…. and explain at a molecular level the etiology of some diseases. However, primarily, these results could indicate new strategies to improve plant iron nutrition in areas where deficiency is prevalent, such as eastern Spain
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy
- natural sciences biological sciences botany
- natural sciences physical sciences optics spectroscopy
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75794 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.