Objective
Basic knowledge of how nerve connections are established during development is essential for the development of clinical therapies in nerve repair. The correct wiring of the nervous system relies on the ability of axons and dendrites to locate and recognize their appropriate synaptic targets during development.
Pathfinding by growing axons is guided by extracelluar guidance factors. Recently, it has become clear that guidance cues can trigger rapid and local synthesis, degradation and endocytosis of protein s, providing a fast and flexible way for growth cones to respond to cues in their microenvironment and adapt their responses to changing conditions.
Key questions raised by these data are
- what particular proteins are translated in response to axon guidance cues
- do different guidance cues elicit dynamic changes in different sets of proteins
- how do these proteins control the chemotactic turning behaviour of a growth cone
The present proposal addresses these questions using a comparative proteomic app roach involving C-terminal puromycyl-tagging of newly synthesized proteins in developing chick retinal growth cones. Once identified, the function of the cue-induced nascent protein(s), will be approached using gene manipulations and protein knock-down experiments in vitro and in vivo in the Xenopus retinotectal system.
Prof. Holts laboratory provides an excellent environment to study all of these aspects of axon guidance both in vitro and in vivo. Moreover, moving to the host institute will complement my scientific training and is essential to achieve my goal of becoming an independent researcher in neurobiology.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences biochemistry biomolecules proteins
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.