Objective
Prokaryotic plankton are the principal mediators of the biogeochemical fluxes in the ocean.The deep ocean has been considered for a long time to be of minor importance for these fluxes.Recent modelling approaches, however, indicate that about 30 percent of the oceanic remineralization is mediated in the meso- and bathypelagic ocean.Prokaryotic rates have rarely been measured in the deep ocean and if they were measured, then under decompressed conditions i.e. surface pressure.There are indications, however, that decompression might severely affect the metabolic rates.In this project, we aim to determine prokaryotic production measurements as a estimation for total prokaryotic biomass under in situ pressure conditions and compared with samples held at decompr essed conditions.Procaryotic production will be measured via incorporation of radiolabelled leucine into prokaryotic biomass and Archaea production via radiolabelled bicarbonate incorporation.Secondly we will determine single cell activity using a modifie d version of the recently developed catalyzed reporter deposition in situ fluorescence hybridization with specific oligonucleotide probes combined with microautoradiography (MICRO-CARD-FISH).Cells taking up radiolabelled substances are surrounded by a silv er-grain halo, which can be quantify by image analysis and epifluorescence microscopy.While this method has been used only qualitatively, the product of size and intensity of the halo should reflect the metabolic activity.Using this approach, we will dete rmine the relative contribution of individual bacterial and Archaea species to the total prokaryotic biomass production in different depth layers of the North Atlantic.Also we will obtain information on the intraspecific differences in the activity of the dominant prokaryotic species.The project will provide essential and novel information on deep water prokaryotic activity and has the potential to alter our view of the role of the ocean in the global carbon cycling.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology environmental engineering energy and fuels biomass energy
- natural sciences biological sciences microbiology bacteriology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
DEN BURG
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.