Objective
Transcriptional regulation defines the identity of pluripotent and differentiated cells. DNA Topoisomerases are enzymes that catalyze the unlinking of the DNA strands by making transient DNA strand breaks and allowing the DNA to rotate around these breaks, a process essential for DNA replication, transcription, chromosome condensation and segregation (Champoux, 2001). A mammalian-specific distinct type of topoisomerase, topoisomerase II beta (TopIIβ), is only abundantly expressed in terminally differentiated cells and is required for neurogenesis (Yang et al, 2000). TopIIβ is proposed to act at the ‘potentiation’ level of transcription to make gene promoters amenable for transcription as a prerequisite for their subsequent development-specific activation. This function could be achieved by regulating higher order chromatin organization of target genomic regions and/or direct interaction with known partners such as condesin and PcG proteins (Lupo R. et al., 2001) and other epigenetic modifiers such as chromatin remodeling complexes. We propose to define the contribution of TopIIβ activity towards epigenetic regulation of neuronal differentiation. We will combine a sophisticated in vitro mouse differentiation system with genome-wide identification of TopIIβ target genes. These will be directly related to existing datasets on development-specific targets of DNA methylation and Polycomb marking (Mohn et al., 2008).To delineate how these local characteristics translate into spatial organization of chromosomal regions we will study genomewide networks of chromosomal interactions for selected target genes using the 4C methodology. This will be functionally complemented by analysis of mouse stem cells lacking topoisomerase II beta protein. We ultimately aim to generate a model of how topoisomerase II beta proteins contribute to epigenetic reprogramming and nuclear reorganization events that take place during cellular differentiation of pluripotent stem cells.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences medical biotechnology cells technologies stem cells
- natural sciences biological sciences genetics epigenetics epigenomes
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IIF-2008
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
4058 BASEL
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.