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The role of lipid membranes in dengue virus assembly

Objective

Viral hemorrhagic fever is a serious health threat mostly caused by the Dengue Virus (DV), which infects 50 to 100 million people worldwide yearly. Aedes spp. mosquitoes, the disease vectors endemic to tropical and subtropical regions, are spreading to temperate regions. A. aegypti, the main vector, is now found in Madeira, Portugal, and A. albopictus, a less frequent albeit potential vector, is present in several European countries. Conditions for a possible dengue outbreak in Europe are now set, only being necessary the mosquitoes to enter in contact with human viral carriers, as recently occurred in the A. albopictus promoted Chikungunya fever 2007 outbreak in Italy. DV infection is therefore a potential clinical problem for Europe, as stated in the EU FP7 Emerging Epidemics program (http://ec.europa.eu/research/health/). Once infected, no specific treatment is available, partially due to the lack of detailed information on the molecular mechanisms of viral assembly. The project intends to fill that gap by studying the viral assembly capsid protein, in biologically relevant conditions, using lipid bilayers mimicking biological membranes and intracellular lipid droplets isolated from hepatic cell lines. The project gathers the complementary expertise of two teams, each having a distinct yet synergistic role. Prof. Da Poian, from the Medical Biochemistry Institute, Federal University of Rio de Janeiro, Brazil, has expertise in cell and molecular biology, NMR and calorimetry studies applied to DV proteins. Prof. Santos, from the Institute of Molecular Medicine, Lisbon, Portugal, is skilled in light scattering spectroscopy and atomic force microscopy applied to lipid bilayers interaction with viral proteins. Thus the project assembles an eclectic gathering of complementary expertise to study the role of lipid membranes in dengue viral assembly. Following this approach, it is expected to open a gateway to the future development of capsid assembly-targeted therapies.

Field of science

  • /medical and health sciences/health sciences/infectious disease/RNA virus
  • /natural sciences/chemical sciences/electrochemistry/bioelectrochemistry/electroporation
  • /natural sciences/biological sciences/molecular biology
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /natural sciences/chemical sciences/analytical chemistry/calorimetry

Call for proposal

FP7-PEOPLE-IOF-2008
See other projects for this call

Funding Scheme

MC-IOF - International Outgoing Fellowships (IOF)

Coordinator

INSTITUTO DE MEDICINA MOLECULAR JOAO LOBO ANTUNES
Address
Avenida Prof Egas Moniz
1649 028 Lisboa
Portugal
Activity type
Research Organisations
EU contribution
€ 137 311,75
Administrative Contact
Margarida Pinto Gago (Dr.)