Objective
The objective of this proposal is to discover components of the ambient temperature perception pathway in Brachypodium distachyon. This will help us understand the way in which crop plants perceive and respond to changes in ambient temperature, including climate change. A gene expression atlas for the major ambient temperature responses in Brachypodium will be created using microarrays, to identify genes that show a continually variable expression across the ambient (i.e. non-stress inducing) temperature range. These experiments will be compared to data from Arabidopsis to identify the level of conservation in temperature perception pathways between monocots and dicots. The promoters of temperature responsive genes identified through this analysis will be fused to the firefly Luciferase gene to develop ambient temperature reporter lines. Selected lines will be used in forward genetic screens (using fast neutron irradiation mutagenesis) to identify key components of the Brachypodium temperature sensing pathway. This analysis will be complemented by functional characterisation of temperature sensing genes identified in parallel screens in Arabidopsis that have been carried in the Wigge lab as well as using the expression atlas data. These reverse genetics approaches will use RNA interference technology to knock-down the activity of the genes of interest. The phenotypes of these plants will be assessed to determine the direct role of each candidate within the temperature perception pathway. Taken together, this project aims to provide novel information about the way in which our major crop plants will respond to changes in ambient temperature by continuing the development of Brachypodium as a model organism for temperate cereals, such as wheat and barley, and identifying genetic components within the monocot temperature perception pathway.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics RNA
- natural sciences earth and related environmental sciences atmospheric sciences climatology climatic changes
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IIF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NR4 7UH NORWICH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.