Objective
Stress is defined as any condition that seriously disturbs physiological and psychological homeostasis and is experienced by every individual throughout life. Exposure to stress can be either beneficial or disruptive in memory formation, and can on the long-term lead to serious neurological disorders.
The aim of this project is to identify protein targets of stress-induced and glucocorticoid receptor-mediated memory formation and impairment. For this, I will investigate stress-induced memory formation or disruption and reveal molecular changes of the proteome at the synapses of the hippocampus, a key structure for cognition and memory formation. In particular, I will use a well-defined learning paradigm, contextual fear conditioning, for assessing hippocampus-dependent, stress-mediated learning and memory. I will couple this behavioural paradigm to state of the art proteomics studies of the synapse, i.e. 2D liquid chromatography (LC-LC) coupled to tandem mass spectrometry (MS-MS) in conjunction with iTRAQ la belling.
This innovative approach is quantitative and can identify hundreds of proteins including membrane proteins, contained in up to four different samples (i.e. experimental conditions). In addition, we will use unique animal models, i.e. inbred and knock out mice, in order to gain a high discriminative power for identifying synaptic proteins that are relevant to the stress response. The outcome of my study will also be instrumental for addressing synaptic plasticity processes in other brain structure s related to stress-related psychopathologies, such as addiction or depression, in which synaptic plasticity changes might also be key processes in establishing pathological states.
Taken together, this analysis will yield novel insight in the molecular mechanisms underlying stress effects, which is of scientific and social relevance. Moreover, this proposal will help me to discover a novel and career opportunity-rich field of proteomics research.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences analytical chemistry mass spectrometry
- medical and health sciences basic medicine neurology
- medical and health sciences basic medicine physiology homeostasis
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.