Objective
Self-incompatibility (SI) in plants prevents self-fertilization and is used to control sexual reproduction. SI is genetically controlled and involves cell-cell recognition between pollen and pistil so incompatible ("self") pollen is rejected. In Papaver rhoeas, SI triggers an intracellular signalling cascade in incompatible pollen, resulting in inhibition of pollen tube growth. The host lab has identified signals and mechanisms involved in SI, and recently published evidence in Nature that programmed cell death (PCD) involving a caspase-like activity is triggered by SI in Papaver pollen.
The scientific aims of this proposal are to identify and characterize key components involved in the early stages of SI-induced PCD in Papaver pollen, which are likely to be important regulators of PCD. The 3 objectives are: A. Characterization of mitochondrial alterations and their role in SI-induced PCD. We will use fluorescent live-cell temporal imaging to investigate mitochondrial permeability and activity. B. Ide ntification and characterization of early caspase-like activities triggered by SI. Caspase-specific peptide inhibitors will be used to identify and monitor different activities in live cells and extracts over time. C. Analysis of early cellular alterations and key targets of caspase activity. We will use a comparative proteomic approach combined with mass spectrometry.
The data generated will contribute considerably to our understanding of: (1) Early events responsible for initiating PCD, particularly the role of the mitochondria. (2) The nature of the initiator and downstream caspase-like activities involved in PCD. (3) The targets of these activities, providing insights into the protease/signalling cascades involved in PCD. Dr Maurice Bosch is a talented and promising scientist. The project will contribute to the transnational mobility of research and address Dr Bosch's career development needs by providing relevant research and complementary training.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences cell biology cell signaling
- natural sciences biological sciences biochemistry biomolecules
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
United Kingdom
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