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Bacterial Synthetic Minimal Genomes for Biotechnology

Objective

BaSynthec will combine computational and experimental biology approaches with novel high-throughput methodologies to reduce and modify à la carte the chromosome of Bacillus subtilis, a genetically tractable bacterium and one of the key microbes used as a Cell Factory in biotechnology. Simpler B. subtilis strains with reduced energy consumption for self maintenance will be designed and constructed by removing some potentially expensive cellular processes. The cells with the lowest experimentally determined waste of energy and with industrially relevant phenotypes will be engineered to reroute the flux devoted to biomass formation through rational modifications of the complex metabolic regulations, and will be used as biotechnological platforms to plug in synthetic modules. For this purpose, BaSynthec will develop a model-driven approach to design and engineer the strains with predetermined features, with a particular focus on unrestricted metabolic activity and the plug-in of synthetic functional modules. This strategy is based on the recent development of two complementary modelling approaches for B. subtilis: i) a genome-scale model of genetic and metabolic regulatory networks associated with a novel method called “Resource Balance Analysis” defining the formal background of model-based approaches for engineering strains; and ii) the development of a new genome-scale metabolic model of B. subtilis which is the most complete and accurate that exists today. Two pathways of high biotechnological relevance will be used for establishing the proof-of-principle of the assembly of functional synthetic modules: i) the vitamin B5 biosynthetic pathway, and ii) the secretion machinery for the export of extra-cellular enzymes. It is anticipated that validated simpler bacterial strains together with the modelling framework generated by BaSynthec will be used as generic biotechnological platforms to better control and exploit cell metabolism in industrial processes.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/chromosome
  • /natural sciences/biological sciences/cell biology/cell metabolism
  • /natural sciences/biological sciences/genetics and heredity/genome
  • /agricultural sciences/agricultural biotechnology/biomass
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

FP7-KBBE-2009-3
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Address
147 Rue De L'universite
75007 Paris Cedex 07
France
Activity type
Research Organisations
EU contribution
€ 893 043
Administrative Contact
Philippe Noirot (Dr.)

Participants (7)

ACADEMISCH ZIEKENHUIS GRONINGEN
Netherlands
EU contribution
€ 326 422
Address
Hanzeplein 1
9713 GZ Groningen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jan Maarten Van Dijl (Prof.)
THE UNIVERSITY OF CHICAGO
United States
Address
S Ellis Ave 5801 Room 503
60637 Chicago Illinois
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Rick Stevens (Prof.)
DSM Nutritional Products Ltd
Switzerland
EU contribution
€ 306 510
Address
Wurmisweg 576
4303 Kaiseraugst
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Zoltán Prágai (Dr.)
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland
EU contribution
€ 607 418
Address
Raemistrasse 101
8092 Zuerich
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Uwe Sauer (Prof.)
UNIVERSITAET GREIFSWALD
Germany
EU contribution
€ 339 203
Address
Domstrasse 11
17489 Greifswald
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Michael Hecker (Prof.)
INRAE TRANSFERT SAS
France
EU contribution
€ 222 854
Address
Rue Du Docteur Finlay 28
75015 Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Marie Dufourcq (Ms.)
NOVOZYMES A/S
Denmark
EU contribution
€ 297 712
Address
Krogshoejvej 36
2880 Bagsvaerd
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Michael Dolberg Rasmussen (Dr.)