Skip to main content
Go to the home page of the European Commission (opens in new window)
English English
CORDIS - EU research results
CORDIS
Content archived on 2024-05-27

Bacterial Synthetic Minimal Genomes for Biotechnology

Objective

BaSynthec will combine computational and experimental biology approaches with novel high-throughput methodologies to reduce and modify à la carte the chromosome of Bacillus subtilis, a genetically tractable bacterium and one of the key microbes used as a Cell Factory in biotechnology. Simpler B. subtilis strains with reduced energy consumption for self maintenance will be designed and constructed by removing some potentially expensive cellular processes. The cells with the lowest experimentally determined waste of energy and with industrially relevant phenotypes will be engineered to reroute the flux devoted to biomass formation through rational modifications of the complex metabolic regulations, and will be used as biotechnological platforms to plug in synthetic modules. For this purpose, BaSynthec will develop a model-driven approach to design and engineer the strains with predetermined features, with a particular focus on unrestricted metabolic activity and the plug-in of synthetic functional modules. This strategy is based on the recent development of two complementary modelling approaches for B. subtilis: i) a genome-scale model of genetic and metabolic regulatory networks associated with a novel method called “Resource Balance Analysis” defining the formal background of model-based approaches for engineering strains; and ii) the development of a new genome-scale metabolic model of B. subtilis which is the most complete and accurate that exists today. Two pathways of high biotechnological relevance will be used for establishing the proof-of-principle of the assembly of functional synthetic modules: i) the vitamin B5 biosynthetic pathway, and ii) the secretion machinery for the export of extra-cellular enzymes. It is anticipated that validated simpler bacterial strains together with the modelling framework generated by BaSynthec will be used as generic biotechnological platforms to better control and exploit cell metabolism in industrial processes.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

You need to log in or register to use this function

Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-KBBE-2009-3
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

CP-FP - Small or medium-scale focused research project

Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
EU contribution
€ 893 043,00
Address
147 RUE DE L'UNIVERSITE
75007 PARIS CEDEX 07
France

See on map

Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data

Participants (7)

My booklet 0 0