Objective
Sub-cellular localisation of RNA has emerged as a key mechanism through which cells become polarized. The localisation of transcripts is an extremely efficient way to target gene products to subcellular compartments or to specific regions of a cell or embryo, making it an important posttranscriptional level of gene regulation. The aim of this project is to perform an RNA-interference (RNAi) screen to identify novel components required for intracellular mRNA transport in Drosophila. A wide range of Drosophila cell-lines will be characterized to select those with asymmetric morphology well suited to visualise localised transcripts. Initially, I will screen for dsRNAs that disrupt RNA localisation as assayed by sensitive fluorescence in situ hybridisation in fixed cells. First, I will examine transcripts that are known to localise in other systems, for example b-actin transcripts that are present at the leading edge of migrating fibroblasts and neurites, and later, identify possible target transcripts expressed i n the selected cells using Drosophila cDNA microarrays. Once a reliable assay has been set up I will begin by inactivating genes already implicated in RNA localisation or microtubule-based transport, and screen more widely when high-throughput methods have been established. Genes that affect localisation without grossly disrupting cytoskeleton organisation will be analysed further genetically and biochemically to examine effects on RNA localisation and development. This RNAi screen will likely lead to the identification of a large number of genes involved in different pathways of RNA localisation and/or individual steps of these pathways. These will include genes already known to be involved in these pathways, but also new candidates. Since the molecular motors involved in RNA localisation are the same motors responsible for transport of other cargos, such as lipid vesicles, the project could contribute to a more integral view on intracellular transport.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences medical biotechnology cells technologies stem cells
- natural sciences biological sciences genetics RNA
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.