Objective
50-70% of the world biological nitrogen fixation, leading to a terrestrial input of 40-50 million tons of nitrogen per year, is carried out by symbiotic nitrogen fixation (SNF), mostly between bacteria of the family Rhizobiaceae and legume plants. A specif ic molecular dialogue takes place during the early steps of the symbiosis, leading to the production of key signalling lipooligosaccharides (Nod factors) by the bacterial partner. Nod factors are essential for the production of specific organs in which SNF takes place, the root nodules, and for Rhizobium infection via infection threads, which form in root hairs and then grow into root and nodule tissues. Profiling the transcriptome of the two model legumes Medicago truncatula and Lotus japonicus led to iden tification of remorin genes that are specifically expressed during symbiotic interactions. In M. truncatula the expression of this gene is induced already 48 hours after Nod factor treatment (A. Niebel, H. Kuster, P. Gamas; non published data); and this up -regulation is even more striking in developing and mature nodules (more than a thousand fold as determined by real-time PCR). Remorins represent a plant specific protein family with similarities to a 30 kD viral movement protein that is involved in macrom olecular trafficking through plasmodesmata. Their recent discovery in lipid rafts together with their structure suggest a potential role in cell to cell communication. Interestingly it was recently shown that remorins are most abundant in meristematic tiss ues and in phloem, which is consistent with a role in development. The aim of the project is to understand the role of remorin during the symbiotic Sinorhizobium meliloti-M. truncatula interaction by: a. Detailed expression analysis b. Generation and analy sis of transgenic roots/plants over-expressing or silencing the remorin gene c. Protein localisation and analysis of lipid raft mediated signalling d. Identification of interacting partners
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences physical sciences optics microscopy fluorescence lifetime imaging
- natural sciences biological sciences biochemistry biomolecules lipids
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CASTANET TOLOSAN
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.