Objective
Phenotype is a manifestation of a complicated relationship between the gene sequence that dictates the “what” of gene expression and regulatory elements that supply the crucial “when, where, and how.” The central components of gene regulation are transcription factors (TFs) – proteins that regulate transcription by binding to DNA regions adjacent to the gene. While TFs recognize specific DNA sequences, we do not fully understand the rules that allow regulators to distinguish among the millions of potentially bind-able sequences in the genome to select the tens of thousands chosen to be bound in vivo. Uncovering these rules is a key step in understanding the developmental and evolutionary processes that shape the observed phenotypic variation. Based on previous experiments we know that, despite the assumption that conserved function will correlate with conservation of TF binding, most TF binding events are species-specific (i.e. not evolutionarily conserved) and are driven by the underlying genetic sequence, not inter-species differences in nuclear environment. In this project, I will experimentally dissect the long-range sequence patterns that govern transcription factor binding. Aim 1: Scan TF binding in closely related species. I will use liver hepatocytes from closely related mouse strains to map the TF binding sites using chromatin immunoprecipitation (ChIP) combined with ultra highthroughput DNA sequencing (ChIP-seq). Aim 2: Identify species-specific TF binding events that do not follow simple binding rules. Find the long-range sequence changes associated with these events. Aim 3: Confirm our findings in related species and formulate rules underlying TF binding. A Marie-Curie International Reintegration Grant would enable this cutting-edge research and help me apply the molecular biology and population genetics skills I obtained during my PhD and postdoc in the United States in an excellent laboratory in the European Union.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences evolutionary biology
- natural sciences biological sciences genetics nucleotides
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-RG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
E20 1JQ LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.