Objective
Understanding the neuronal mechanisms mediating emotional information processing in the amygdala will require identification of its main cell types and knowledge of their participation in the intrinsic circuitry of this region. Amygdala pyramidal neurons a re spiny projection neurons that use glutamate as an excitatory neurotransmitter.
In contrast, most non-pyramidal neurons in the amygdala are aspiny interneurons that use GABA as an inhibitory neurotransmitter. Very few studies linking physiological and morphological properties of the various cell types of the amygdala have been done. The precise identification of the main cell types in the amygdala and knowledge of their participation in the intrinsic circuitry of this region is an essential pre-requisite to understand the neuronal mechanisms mediating the diverse roles of the amygdala subsystems.
The aim of this project is to characterise some electrophysiological and pharmacological properties of neurochemically and morphologically identified interneurones in the amygdala. This will be a multidisciplinary study in which intracellular recordings followed by intracellular filling will be combined with immunocytochemical characterisation and cellular reconstruction.
For this purpose, a transgenic mouse line expressing a green fluorecescent protein (GFP) under the control of the GAD65 (GAD: glutamate decarboxilase enzyme involved in the synthesis of GABA) promoter will be used. After the single, double or triple intracellular recordings, sections will be fixed and re-sectioned. Immunoreactivity for neurotransmitter receptors, calcium binding proteins and neuropeptides will be visualized by immunocytochemistry and the sections will be processed.
The axonal and dendritic patterns of each neurone will be then analysed at high magnification and reconstructed. Structural properties of interneuron to principal cell and principal cell to interneuron inputs will also be investigated by electron microscopic analysis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine anatomy and morphology
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- medical and health sciences clinical medicine psychiatry posttraumatic stress disorder
- engineering and technology electrical engineering, electronic engineering, information engineering information engineering telecommunications telecommunications networks mobile network
- natural sciences computer and information sciences data science data processing
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.