Objective
The ubiquitin-like proteins SUMO-1 and SUMO-2 are covalently conjugated to a wide range of target proteins where they serve as subunits of complex protein associations (sub-proteomes). These covalent modifications play important roles in cellular processes such as transport, regulation of transcription, signalling, and others. In this proposed project I would like to take advantage of several advanced techniques in molecular cell biology and mass spectrometry.
In the first part of my study I will define the proteins covalently interacting with each of the SUMO paralogues in human cells. To achieve this I will use cell lines with integrated versions of TAP-tagged SUMO-1 and -2, which will allow me to recover them together by affinity with other tightly bound proteins. I will then determine how the abundance of proteins modified by SUMO changes during the course of the cell cycle and in response to stress conditions such as UV, heat and hypoxia.
Quantitative changes in modified proteins will be determined by mass spectrometry using SILAC (Stable Isotope Labelling by Amino acids in Cell culture) technique. These experiments will add significant, new and valuable data concerning SUMO sub-proteomes. Obtained results should allow for creation of complex and hopefully quite clear picture of the relationships among all the analysed proteins.
Summarizing, these data should help our understanding of the many cellular processes not necessarily directly involved in the SUMO sub-proteomes. Lists of SUMO targets will be made available to the scientific community so that groups with an interest in a particular protein can determine the role of SUMO modification in its function.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences data science
- natural sciences computer and information sciences knowledge engineering ontology
- natural sciences chemical sciences organic chemistry amines
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
DUNDEE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.