Objective Notch signalling plays a critical role in the intercellular communication involved in cell fate determination, stem cell potential and differentiation in many different tissues of multicellular organisms.Therefore, the downstream responses following the activation of Notch must be flexible and context-sensitive so that appropriate intracellular responses are mounted in any given situation. This tightly regulated process makes Notch signalling an interesting research topic.Here we describe a screen with an RNAi library to identify novel genes involved in the Notch signalling pathway. Previous results have shown that the RNAi library is successful in the identification of genes using a gain-of-function screen. As activation of Notch1 in human keratinocytes will result in senescence of these cells, we will screen for genes that can rescue this phenotype.The obtained results can provide important information on the regulation of cell differentiation and tumorigenesis. Fields of science medical and health sciencesmedical biotechnologycells technologiesstem cells Keywords Notch Notch differentiation RNAi differentiation tumorigenesis Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Topic(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Call for proposal FP6-2002-MOBILITY-5 See other projects for this call Funding Scheme EIF - Marie Curie actions-Intra-European Fellowships Coordinator UNIIVERSITY OF LAUSANNE Address Switzerland See on map EU contribution € 0,00
