Objective
Nuclear pore complexes (NPCs) are large multi-protein structures (~125 MDa) spanning the double membrane of the nucleus and acting as the only gateway of nucleocytoplasmic transport. The three dimensional (3D) architecture of the NPC, its role in trafficking of proteins and RNA, and its molecular composition (nucleoporins 'Nups') have been extensively studied.
Nonetheless, to understand how the NPC mediates nucleocytoplasmic exchange requires the independent characterization of its molecular components at atomic resolution and a detailed description of physical interactions between nucleoporins in the NPC assembly. Crystal structures of three nucleoporin domains have been resolved (Nup98, hNup133, Nup159), although no atomic model for any sub-complex of N ups is yet available.
This proposal aims to elucidate the structure of the nuclear pore subcomplex p62, a biomedical agent essential to signal-mediated nucleocytoplasmic transport, using 'bi-cistronic multiple co-expression' for in vitro complex reconstitution and X-ray crystallography. This accomplishment will contribute to the construction of parts of the NPC at atomic detail essential to understand nucleocytoplasmic trafficking and to establish basic knowledge into human diseases caused by disruption of transport pathways (objective of the LifeSciHealth Priority Programme of Framework 6).
The applicant, of Spanish nationality, will accomplish this task under the supervision of Dr. Olga Mayans at the renowned Swiss institute Biozentrum (in compliance with the section 2.3.2.1 of the WP HRM). The multidisciplinary and advanced training received by the applicant will complement and expand her technical experience and research management capabilities to initiate an independent career in Molecular Structural Biology.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering crystals
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences molecular biology structural biology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.