Objective
With this proposal, funding is applied to conduct research on a membrane-bound receptor p58/ERGIC-53 at the Karolinska Institutet in Stockholm, Sweden. p58/ERGIC-53 is a calcium-dependent, mannose-selective animal lectin that acts as a glycoprotein cargo receptor in the endoplasmic reticulum (ER) and mediates specific transport of target proteins to the Golgi complex.
Mutations in p58/ERGIC-53 cause a bleeding disorder due to deficiency of blood coagulation factors V and VIII, which are known to be secreted specifically by p58/ERGIC-53.
The crystal structure of the carbohydrate recognition domain of p58/ERGIC-53 is known, but structural information on the full-length protein, as well as complexes with cargo proteins and coactivators, are needed to understand the mechanisms of protein transport along the ER-Golgi secretory pathway.
The aim of this study is to determine crystal structures of longer constructs of p58/ERGIC-53 and of complexes with mannose and high-mannose cargo proteins. In addition, a complex with a coactivator MCFD2, which is an EF-hand protein, will be pursued.
These data are expected to provide us with the structural basis for understanding receptor-cargo interactions in this family of membrane-bound receptors. The host laboratory has excellent facilities for molecular biology, protein production and purification as well as X-ray crystallography. I myself as the applicant have thorough knowledge in these methods and especially in crystal structure determination.
Thus, the goals set are possible to reach within a time frame of two years, for which funding is applied. The planned visit will be the second post-doctoral period outside the home country for theme and will serve as an important step towards an independent academic career.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering crystals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- natural sciences biological sciences molecular biology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
STOCKHOLM
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.