Objective
Rho-family GTPases are signalling molecules that play a critical role during thymocyte development and T cell activation. The biochemical pathways regulated by Rho GTPases in lymphocytes are not at all understood although their importance is clear.
Recently the generation of mice deficient in Rho-family GTPases, their regulators and effectors have revealed functions for these GTPases at multiple regulatory levels, including the coordination of cytoskeletal change, activation of kinase cascades, stimulation of calcium fluxes, and the induction of gene expression. Information about the activation of Rho-family GTPases in T cells has been mainly provided by biochemical experiments, which allow only a global measure of GTPase activation.
However, it remains largely unknown when and where Rho-family GTPases are activated during T cell activation induced by various stimuli. Here we propose to use fluorescent resonance energy transfer (FRET)-based sensors to report the spatio-temporal activation of Rho-family GT Pases in living T cells in response to diverse stimuli such as TCR triggering, integrin engagement or chemokine stimulation.
We aim to perform this both in wild-type T cells and in T cells deficient in various guanine nucleotide exchange factors (GEFs) that are believed to activate Rho-family GTPases. In summary, this project will provide a better understanding of when, where and how different GTPases are activated in T cells in response to different signalling receptors.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors
- natural sciences biological sciences genetics nucleotides
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.