Objective Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic Escherichia coli (EHEC) are closely related diarrheagenic pathogens that are important causes of morbidity and mortality worldwide, especially in young children. EPEC and EHEC are known as attaching and effacing pathogens because they intimately adhere to gut enterocytes and instigate effacement of brush border microvilli. Injection of bacterial effector proteins via a type III secretion system (T3SS) is an integral part of the EPEC and EHEC infection strategy. One such effector, EspF, binds and induces degradation of the anti-apoptotic protein AbcF2, however, although infected cells show early apoptotic symptoms, they do not present late apoptosis signs. The T3SS effectors NleH1 and NleH2 were recently found to have a broad range anti-apoptotic activity which may neutralise the effect of EspF and promote cell survival. In this proposal we wish to investigate the anti-apoptotic activity of NleH, in particular its binding to the endoplasmic reticulum (ER) anti-apoptotic protein Bax inhibitor-1 (BI-1) and to PDZ domain-containing protein/s. Fields of science social sciencessociologydemographymortalitynatural sciencesbiological sciencesbiochemistrybiomoleculesproteins Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2009-IIF - Marie Curie Action: "International Incoming Fellowships" Call for proposal FP7-PEOPLE-2009-IIF See other projects for this call Funding Scheme MC-IIF - International Incoming Fellowships (IIF) Coordinator IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE EU contribution € 182 103,20 Address SOUTH KENSINGTON CAMPUS EXHIBITION ROAD SW7 2AZ LONDON United Kingdom See on map Region London Inner London — West Westminster Activity type Higher or Secondary Education Establishments Administrative Contact Brooke Alasya (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
