Objective
New techniques are urgently needed for the analysis of peptides in biological samples, for instance in the field of searching for tumour biomarkers or within the context of drug discovery.
Currently the combination of efficient microseparations and sensitiv e laser-induced fluorescence (LIF) detection is widely used. Unfortunately, LIF usually requires derivatization of the analytes, giving rise to complications especially when dealing with extremely low analyte concentrations in the presence of an excess of chemical interferences. Furthermore, biological samples often show a strong fluorescent background.
We will develop two alternatives for LIF, both based on time-resolved luminescence in the millisecond range.
- Quenched laser-induced phosphorescence detection, applicable without chemical derivatization. It will be coupled to miniaturized separation systems for biomarker detection.
- Time-resolved energy transfer luminescence detection for homogeneous bioassays coupled on-line to liquid chromatography, a zero-background detection technique that directly provides information about the biological activity of analytes.
The postdoctoral fellow (experienced in bioanalytical separations) will receive a theoretical and practical training in lasers, novel spectroscopic principles and optics; subsequently emphasis will be on the coupling of time-resolved luminescence with liquid separation methods. The host group participates in the Laser Centre Vrije Universiteit Amsterdam (LCVU, recognized as large scale research facility by the EU), and collaborates with VU Medical Centre, which warrants the efficient exchange of expertise and instrumentation.
Weekly research seminars are given in English, discussing the most recent developments in adjacent fields of analytical chemistry, spectroscopy and biomedical sciences. The fellow will also attend a broad range of symposia and specialty courses to deepen her knowledge and further develop her academic skills.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine pharmacology and pharmacy drug discovery
- natural sciences chemical sciences analytical chemistry
- natural sciences biological sciences biochemistry biomolecules
- natural sciences physical sciences optics laser physics
- natural sciences physical sciences optics spectroscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
AMSTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.